PMID: 11342100May 9, 2001Paper

The rainbow trout metallothionein-B gene promoter: contributions of distal promoter elements to metal and oxidant regulation

Biochimica Et Biophysica Acta
S L SamsonL Gedamu

Abstract

In this report, the contributions of the distal 5'-regulatory sequences of the rainbow trout (Oncorhynchus mykiss) metallothionein (tMT)-B gene promoter (-738 to +5) were studied. Transfection of the -738 promoter fragment in a rainbow trout hepatoma cell line (RTH-149) resulted in 4- to 5-fold greater activity compared to the proximal -137 promoter region. Mutation of the proximal MREa abolishes the basal activity of the -738 fragment indicating that the distal regulatory elements require a cooperative interaction with MREa. However, the fragments containing both distal MREs, c and d (positioning -570 and -680, respectively), or MREc alone could confer basal and metal-induced activity when fused to the TATA box. This suggests that these distal elements are functional and therefore may play a role as basal elements in their natural state. The trout MT genes are also induced by oxidants including H2O2, tBHP and tBHQ. The larger promoter fragment -738 responds to H2O2, while the -137 fragment does not. However, fusion of the isolated MREc fragment (-648 to -533) in its native orientation, upstream of the -137 promoter elicits a response to H2O2, although no response is seen with MREc in reverse. These data suggest that this dista...Continue Reading

References

Feb 14, 1992·Archives of Biochemistry and Biophysics·H Fliss, M Ménard
Dec 11, 1992·Toxicology and Applied Pharmacology·J W BaumanC D Klaassen
Apr 1, 1992·Toxicology and Applied Pharmacology·K S MinK Tanaka
Jan 1, 1992·Archives of Toxicology·M SatohN Imura
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R S FrilingV Daniel
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J J Schroeder, R J Cousins
Jul 30, 1990·Biochimica Et Biophysica Acta·M ZafarullahL Gedamu
Oct 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R J Cousins, A S Leinart
Mar 1, 1989·Molecular and Cellular Biology·V C Culotta, D H Hamer
Sep 1, 1989·Gene·L P Fernando, G K Andrews
Jan 1, 1987·Experientia. Supplementum·P F SearleR D Palmiter
Nov 15, 1988·Biochemistry·J H Kägi, A Schäffer
Feb 1, 1987·Molecular and Cellular Biology·J R de WetS Subramani
Oct 1, 1985·Molecular and Cellular Biology·M KarinG Wong
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G W StuartR D Palmiter
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·T Prestera, P Talalay
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K T TamaiD J Thiele
Jan 15, 1998·Progress in Nucleic Acid Research and Molecular Biology·S L Samson, L Gedamu
Sep 28, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T P DaltonM J Carvan

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Citations

Nov 5, 2013·Fish & Shellfish Immunology·Øystein Evensen, Jo-Ann C Leong
Jun 20, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Susanna AiraksinenMikko Nikinmaa
Aug 3, 2010·Folia Histochemica Et Cytobiologica·Agnieszka GomulkiewiczPiotr Dziegiel
Mar 7, 2006·Biochemical and Biophysical Research Communications·Hongwei RenMingming Chu
Nov 29, 2005·Biochimica Et Biophysica Acta· CheungKing Ming Chan
Mar 6, 2007·The International Journal of Biochemistry & Cell Biology·Pengfei HeHongwei Ren
Dec 14, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nic R BuryChrister Hogstrand
Jun 28, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Dian GaoPin Nie
Jan 22, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Laura VerganiGabriella Gallo

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