PMID: 2124544Nov 26, 1990Paper

The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library

FEBS Letters
K NagataY Nozawa

Abstract

A novel low Mr GTP-binding protein cDNA was isolated from a rat megakaryocyte cDNA library with a synthetic oligonucleotide probe corresponding to an 8-amino acid sequence specific for c25KG, a GTP-binding protein previously isolated from human platelet cytosol fraction [(1989) J. Biol. Chem. 264, 17000-17005]. The cDNA has an open reading frame encoding a protein of 221 amino acids with a calculated Mr of 25068. The protein is designated as ram (ras-related gene from megakaryocyte) protein (ram p25). The amino acid sequence deduced from the ram cDNA contains the consensus sequences for GTP-binding and GTPase domains. ram p25 shares about 23%, 39% and 80% amino acid homology with the H-ras, smg25A and c25KG proteins, respectively. The 3.5-kb ram mRNA was detected abundantly in spleen cells.

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Citations

Sep 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K NagataY Nozawa
Nov 26, 1999·Gene·T TolmachovaM C Seabra
Sep 23, 2003·Biochimica Et Biophysica Acta·Karine A Cohen-SolalSuzie Chen
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y T ChenH P Moore
Nov 15, 2008·The Journal of Biological Chemistry·Joseph T RolandJames R Goldenring
Jan 1, 1992·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·K NagataY Nozawa
Feb 28, 2002·Molecular and Cellular Biology·Zhaohong YiTetsuro Izumi
Jul 16, 2003·Molecular and Cellular Biology·Taruho S KurodaMitsunori Fukuda
Apr 6, 2007·Genetics·Jun ZhangMatthew P Scott
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·S M WilsonN A Jenkins
Mar 27, 2001·The Journal of Cell Biology·E K HaddadP A Henkart
Dec 31, 2003·The Journal of Biological Chemistry·Ryutaro ShirakawaHisanori Horiuchi
Jan 15, 2004·The Journal of Biological Chemistry·Mitsunori FukudaAkitsugu Yamamoto
Aug 2, 2006·The Journal of Biological Chemistry·Hirokazu KondoHisanori Horiuchi
Mar 27, 2001·The Journal of Cell Biology·A N HumeM C Seabra
Oct 13, 1995·The Journal of Biological Chemistry·M C SeabraJ S Anant
Mar 25, 2004·Journal of Cell Science·Akane ImaiMitsunori Fukuda

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