The Ramazzini Institute 13-week pilot study on glyphosate and Roundup administered at human-equivalent dose to Sprague Dawley rats: effects on the microbiome

Environmental Health : a Global Access Science Source
Qixing MaoJianzhong Hu

Abstract

Glyphosate-based herbicides (GBHs) are broad-spectrum herbicides that act on the shikimate pathway in bacteria, fungi, and plants. The possible effects of GBHs on human health are the subject of an intense public debate for both its potential carcinogenic and non-carcinogenic effects, including its effects on microbiome. The present pilot study examines whether exposure to GBHs at doses of glyphosate considered to be "safe" (the US Acceptable Daily Intake - ADI - of 1.75 mg/kg bw/day), starting from in utero, may modify the composition of gut microbiome in Sprague Dawley (SD) rats. Glyphosate alone and Roundup, a commercial brand of GBHs, were administered in drinking water at doses comparable to the US glyphosate ADI (1.75 mg/kg bw/day) to F0 dams starting from the gestational day (GD) 6 up to postnatal day (PND) 125. Animal feces were collected at multiple time points from both F0 dams and F1 pups. The gut microbiota of 433 fecal samples were profiled at V3-V4 region of 16S ribosomal RNA gene and further taxonomically assigned and assessed for diversity analysis. We tested the effect of exposure on overall microbiome diversity using PERMANOVA and on individual taxa by LEfSe analysis. Microbiome profiling revealed that low-dos...Continue Reading

References

Jun 15, 2000·Regulatory Toxicology and Pharmacology : RTP·G M WilliamsI C Munro
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·E SchönbrunnW Kabsch
Dec 8, 2005·Applied and Environmental Microbiology·Catherine Lozupone, Rob Knight
Nov 14, 2006·Journal of Microbiological Methods·Kurt Houf, Roger Stephan
Dec 22, 2006·Nature·Peter J TurnbaughJeffrey I Gordon
Dec 2, 2008·Nature·Peter J TurnbaughJeffrey I Gordon
Jun 6, 2009·The Journal of Physiology·Peter J Turnbaugh, Jeffrey I Gordon
Jun 23, 2009·Toxicology·Céline GasnierGilles-Eric Séralini
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Aug 17, 2010·Bioinformatics·Robert C Edgar
Sep 2, 2010·World Journal of Gastroenterology : WJG·Carlos W NossaZhiheng Pei
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rochellys Diaz HeijtzSven Pettersson
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Nov 29, 2011·Archives of Toxicology·Marco Aurelio RomanoClaudio Alvarenga de Oliveira
Dec 29, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Amy Lavin WilliamsJohn M DeSesso
Mar 14, 2012·Nature Reviews. Genetics·Ilseung Cho, Martin J Blaser
Sep 28, 2012·Journal of Agricultural and Food Chemistry·Stephen O DukeRaymond Hammerschmidt
Feb 7, 2013·Trends in Neurosciences·Jane A Foster, Karen-Anne McVey Neufeld
Aug 6, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·T G Dinan, J F Cryan
Feb 20, 2014·Environmental Toxicology and Chemistry·Michael S MajewskiPaul D Capel
Jul 8, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Isis CoalovaGabriela Chaufan
Oct 2, 2014·The Journal of Clinical Investigation·W H Wilson Tang, Stanley L Hazen
Jan 7, 2015·Disease Models & Mechanisms·Thi Loan Anh NguyenJeroen Raes
Jan 31, 2015·Current Opinion in Pediatrics·Jerrold J Heindel, Laura N Vandenberg
Feb 6, 2015·Microbial Ecology in Health and Disease·Juan Miguel RodríguezMaria Carmen Collado
Aug 20, 2015·Nutrients·María Carmen CenitYolanda Sanz
Sep 2, 2015·FEMS Microbiology Reviews·Debby LaukensPeter Vandenabeele
Nov 19, 2015·The Science of the Total Environment·Molli M NewmanJoseph W Kloepper

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Citations

Apr 17, 2019·PloS One·Nicolas BlotHélène Delatte
Jan 22, 2020·Rapid Communications in Mass Spectrometry : RCM·Katarina Fritz-WallaceUlrike Rolle-Kampczyk
Jul 21, 2020·Scientific Reports·Marcella SpinaciGiovanna Galeati
Oct 16, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Carlo CaiatiMario Erminio Lepera
May 31, 2018·Environmental Health : a Global Access Science Source·Philip J Landrigan, Fiorella Belpoggi
May 12, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Karen ChiuWenyan Mei
Jun 27, 2019·BMJ : British Medical Journal·John D Potter
Apr 3, 2020·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Melody L HartzlerLara Zakaria
May 11, 2019·Nutrients·Fernando RizzelloPaolo Gionchetti
Aug 24, 2019·Neurotoxicology·Lola Rueda-RuzafaDiana Cardona
Dec 30, 2020·Biomolecules·Clémence RivesWalter Wahli
Mar 20, 2021·Environment International·Maite De MaríaNancy D Denslow
Apr 6, 2021·Frontiers in Endocrinology·Renata Marino RomanoMarco Aurelio Romano
Jul 4, 2020·Molecular and Cellular Endocrinology·Xiao-Min RenBruce Blumberg
May 25, 2021·Frontiers in Public Health·Francesca TruzziGiovanni Dinelli
Jul 27, 2021·Frontiers in Endocrinology·María Mercedes MilesiJorgelina Varayoud
Jul 12, 2021·Toxicology·Ambra MaddalonEmanuela Corsini
Aug 4, 2021·Environmental Health : a Global Access Science Source·Charles BenbrookRobin Mesnage
Aug 3, 2021·Environmental Research·Corina LesseurJia Chen
Aug 21, 2021·Environmental Health : a Global Access Science Source·Colin L SoskolneLisa A Bero

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Methods Mentioned

BETA
PCR
electrophoresis
16S sequencing
enzyme-linked immunosorbent assay

Software Mentioned

uclust
fastx
LEfSe
QIIME
seqtk
R package vegan
PyNAST

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