The rapid detection method by polymerase chain reaction for minute intestinal trematodes: Haplorchis taichui in intermediate snail hosts based on 18s ribosomal DNA

Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology
Thapana ChontananarthThanawan Tejangkura

Abstract

The minute intestinal trematode, Haplorchis taichui, is an important parasite species that can infect humans and other mammals. This study investigated the outbreak of H. taichui in thiarid snails in the lower part of the Chao Phraya Basin, Thailand by employing morphological and molecular-based methods. In development of a specific primer of H. taichui, the PCR reaction was conducted with no cross-reaction to their hosts and other related trematode species. The highest level of sensitivity that could be amplified was 0.50 ng/μl and this was detected with only one egg in the sample. In terms of the epidemic results, the parapleurolophocercous cercaria infected only two species of thiarid snails (Melanoides tuberculata and Tarebia granifera) with an overall prevalence of 3.80% (23/605). The process of molecular identification revealed positive results indicating that eleven from twenty-three of parapleurolophocercous cercariae specimens in the lower part of the Chao Phraya Basin were H. taichui. In conclusion, this study has developed a rapid detection method, which can discriminate H. taichui from other parapleurolophocercous cercaria in intermediate snail hosts with a high level of sensitivity. Moreover, the high proportion of...Continue Reading

References

Jan 30, 2004·European Journal of Clinical Nutrition·T E FoxS J Fairweather-Tait
Jun 24, 2004·Diseases of Aquatic Organisms·R DzikowskiI Paperna
Aug 5, 2008·Experimental Parasitology·Supap SaenphetJong-Yil Chai
Sep 30, 2010·The Korean Journal of Parasitology·Dorn WatthanakulpanichYukifumi Nawa
Jan 15, 2011·The Korean Journal of Parasitology·Thapana Chontananarth, Chalobol Wongsawad
Dec 4, 2012·Acta Tropica·Annette S BoerlageMart C M de Jong
Mar 8, 2013·The Korean Journal of Parasitology·Han-Jong RimSithat Insisiengmay
Apr 10, 2013·Parasitology Research·Annette S BoerlageMart C M de Jong
Apr 27, 2013·Asian Pacific Journal of Tropical Biomedicine·Thapana Chontananarth, Chalobol Wongsawad
Feb 12, 2014·The Korean Journal of Parasitology·Somchai PinlaorPaiboon Sithithaworn
Jul 19, 2015·Parasites & Vectors·Markéta OndračkováPavel Jurajda
Mar 14, 2017·The Korean Journal of Parasitology·Thapana ChontananarthCherdchay Chimburut

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