PMID: 6981412Jun 1, 1982Paper

The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices

The Biochemical Journal
M D ScawenT Atkinson

Abstract

3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two triazine dye-Sepharose matrices. Recoveries of both enzymes were in excess of 60%. Malate dehydrogenase could also be purified by a combination of triazine dye affinity chromatography and gel filtration on Ultrogel AcA-44, but this offered no significant advantage over the purely affinity procedure.

Citations

Sep 27, 2005·International Journal of Biological Macromolecules·Jingling ShentuZhishen Jia
Jan 1, 1988·Critical Reviews in Biotechnology·Y D Clonis
Aug 26, 1983·Journal of Chromatography·D A SmallC R Lowe
Dec 1, 1995·Journal of Chromatography. a·N E Labrou, Y D Clonis
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·V BohácováA Ziegelhöffer
May 1, 1988·Journal of Biochemical and Biophysical Methods·L MiribelP Arnaud
Dec 1, 1985·Journal of Steroid Biochemistry·J A PinedaJ C Warren
Dec 1, 1989·Journal of Steroid Biochemistry·J A PinedaJ C Warren
Jan 1, 1988·Journal of Biomedical Engineering·E A Hall

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