PMID: 11012679Sep 30, 2000Paper

The rat kidney acylase I, characterization and molecular cloning. Differences with other acylases I

European Journal of Biochemistry
Thierry GiardinaAntoine Puigserver


The soluble acylase I from rat kidney was purified to homogeneity using a five-step procedure. As the resulting protein was found to have a relative molecular mass of 125 kDa based on size-exclusion chromatography and 44 kDa based on SDS/PAGE, the native protein was taken to consist of three subunits. The amino-acid sequence of a peptide resulting from limited proteolysis of the polypeptide chain with proteinase K, which was determined by microsequencing (RHEFHALRAGFALDEGLA), was found to be very similar to the corresponding sequence of porcine kidney acylase I. However, as N-furyl-acryloyl-L-methionine, a synthetic substrate for porcine acylases, was not hydrolyzed by the rat enzyme, it was suggested that the polypeptide chain might differ in other respects from those of the other acylases I. A full length cDNA coding for the rat kidney acylase I was therefore isolated and found to contain a 1224-bp open reading frame encoding a protein consisting of 408 amino-acid residues, which corresponded to a calculated molecular mass of 45 847 Da per subunit. The deduced amino-acid sequence showed 93.6% and 87.2% identity with that of the human liver and porcine kidney, respectively.


Sep 14, 1976·Biochimica Et Biophysica Acta·W Kördel, F Schneider
May 1, 1977·Zeitschrift Für Naturforschung. Section C: Biosciences·W Kördel, F Schneider
Jan 1, 1978·Advances in Experimental Medicine and Biology·A J PuigserverJ R Whitaker
Dec 1, 1992·Journal of Biochemistry·M MittaS Tsunasawa
Dec 1, 1992·Biological Chemistry Hoppe-Seyler·M JakobK H Röhm
May 1, 1987·Biological Chemistry Hoppe-Seyler·H G Löffler, F Schneider
Feb 9, 1994·Molecular and Cellular Biochemistry·M IbrahimA M Kidwai
Jan 1, 1994·Advances in Pharmacology·M W Anders, W Dekant
Aug 19, 1993·Biochimica Et Biophysica Acta·M MittaS Tsunasawa
Jul 22, 1998·Chemical Research in Toxicology·V UttamsinghM W Anders

❮ Previous
Next ❯


Mar 31, 2005·Biochemical and Biophysical Research Communications·Sameh HergaThierry Giardina
Jul 16, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Josette PerrierAntoine Puigserver
Apr 17, 2007·FEBS Letters·Sergey RyazantsevAlexander Pushkin
Jan 17, 2020·Critical Reviews in Toxicology·Patrick E Hanna, M W Anders
Oct 3, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Debra NewmanAlexander Pushkin
Mar 18, 2008·Biochemistry·Holger A LindnerTraian Sulea
Dec 5, 2008·Journal of Agricultural and Food Chemistry·William MullenAlan Crozier

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.