The rate and character of spontaneous mutation in Thermus thermophilus.

Genetics
Reena R MackwanDennis W Grogan

Abstract

Selection of spontaneous, loss-of-function mutations at two chromosomal loci (pyrF and pyrE) enabled the first molecular-level analysis of replication fidelity in the extremely thermophilic bacterium Thermus thermophilus. Two different methods yielded similar mutation rates, and mutational spectra determined by sequencing of independent mutants revealed a variety of replication errors distributed throughout the target genes. The genomic mutation rate estimated from these targets, 0.00097 +/- 0.00052 per replication, was lower than corresponding estimates from mesophilic microorganisms, primarily because of a low rate of base substitution. However, both the rate and spectrum of spontaneous mutations in T. thermophilus resembled those of the thermoacidophilic archaeon Sulfolobus acidocaldarius, despite important molecular differences between these two thermophiles and their genomes.

References

Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Jan 1, 1974·Journal of Biochemistry·T Oshima, K Imahori
Jul 26, 1996·The Journal of Biological Chemistry·R S LaskenA Rashtchian
Jun 1, 1996·Applied and Environmental Microbiology·A YamagishiT Oshima
Feb 25, 1999·Mutation Research·D SagherB Strauss
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·W A Rosche, P L Foster
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D W GroganJ W Drake
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·Q SheJ Van der Oost
Sep 27, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Y KawarabayasiH Kikuchi
Apr 6, 2002·FEMS Microbiology Letters·Michelle S Reilly, Dennis W Grogan
Jun 5, 2002·Annual Review of Biochemistry·Myron F Goodman
Aug 5, 2003·Journal of Bacteriology·Pablo CastánJosé Berenguer
Mar 17, 2004·Journal of Molecular Biology·Gillian ShuttleworthBernard A Connolly
Apr 6, 2004·Nature Biotechnology·Anke HenneHans-Joachim Fritz
Apr 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Magnus LundgrenRolf Bernander
Apr 2, 2005·Genetics·John W Drake, Charles B C Hwang
Jul 5, 2005·Journal of Bacteriology·Lanming ChenRoger A Garrett
Nov 2, 2005·Nature Reviews. Microbiology·Michael M Cox, John R Battista
Mar 21, 2006·Trends in Biochemical Sciences·Miguel Garcia-Diaz, Thomas A Kunkel
Jun 3, 2006·Journal of Bacteriology·Peter Redder, Roger A Garrett
Dec 22, 2006·PloS One·Sebastian BreuertJörg Soppa
Nov 1, 1943·Genetics·S E Luria, M Delbrück

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Citations

Mar 24, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Fyodor A Kondrashov, Alexey S Kondrashov
Dec 12, 2012·MBio·Ariel D WeinbergerEugene V Koonin
Dec 5, 2014·Extremophiles : Life Under Extreme Conditions·Derrick L LewisRobert M Kelly
Dec 13, 2005·PLoS Pathogens·Stanley G SawickiStuart G Siddell
Oct 31, 2016·Trends in Parasitology·Sutherland K Maciver
Apr 19, 2011·Molecular Biology and Evolution·Mathieu Groussin, Manolo Gouy
Apr 11, 2019·Molecular Genetics and Genomics : MGG·Yoichiro TogawaKeiichiro Hiratsu

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