PMID: 9192617Jun 24, 1997Paper

The rational design and construction of a cuboidal iron-sulfur protein

Proceedings of the National Academy of Sciences of the United States of America
C D ColdrenJ P Caradonna

Abstract

Rational protein design is an emerging approach for testing general theories of protein chemistry through the creation of new structures and functions. Here we present the first successful introduction by rational design of a [Fe4S4] cuboidal cluster into the hydrophobic core of Escherichia coli thioredoxin, a protein normally devoid of metal centers. Cuboidal [Fe4S4] is one of the stable forms of self-assembled iron-sulfur clusters that are thought to represent some of the earliest evolved biological redox centers. [Fe4S4] clusters have been recruited for use in a variety of proteins whose functions are central to many of the major biochemical processes ranging from simple soluble electron-transfer agents, to membrane-bound components of electron-transfer chains, to electron reservoirs in complex metalloenzymes such as nitrogenase. By situating an [Fe4S4] cluster into a protein environment not previously adapted by evolution we can explore the factors by which their activity is modulated by the protein matrix.

References

Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E AdmanL H Jensen
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R W KriwackiJ P Caradonna
Mar 1, 1992·Trends in Biochemical Sciences·J N HigakiC S Craik
Mar 5, 1990·Journal of Molecular Biology·S K KattiH Eklund
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Russel, P Model
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·C W CarterL H Jensen
Nov 10, 1995·Science·J W BrysonW F DeGrado
Aug 1, 1995·Current Opinion in Biotechnology·D J Matthews
Jul 1, 1995·Trends in Biochemical Sciences·L Regan
Feb 1, 1994·Molecular Biotechnology·D S TawfikB S Green
Dec 1, 1995·Protein Science : a Publication of the Protein Society·S G IwagamiL D Eltis
Aug 1, 1996·Current Opinion in Biotechnology·H W Hellinga
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·B R GibneyP L Dutton
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·A L PintoJ P Caradonna
Nov 7, 1996·Chemical Reviews·P. J. StephensA. Warshel

❮ Previous
Next ❯

Citations

Oct 7, 1998·Protein Science : a Publication of the Protein Society·E Farinas, L Regan
Sep 11, 2002·Journal of Molecular Biology·Christopher M SummaWilliam F DeGrado
Jul 4, 1998·Trends in Biotechnology·A E NixonS J Benkovic
Sep 30, 1998·Current Opinion in Biotechnology·D E BensonH W Hellinga
Aug 28, 1998·Current Opinion in Biotechnology·J Wilken, S B Kent
Sep 8, 1998·Current Opinion in Structural Biology·L Regan, J Wells
Sep 8, 1998·Current Opinion in Structural Biology·J R Desjarlais, N D Clarke
Jul 17, 1998·Current Opinion in Chemical Biology·B R GibneyP L Dutton
Jul 17, 1998·Current Opinion in Chemical Biology·M K Johnson
Jan 23, 1999·Current Opinion in Chemical Biology·G A Lazar, T M Handel
Nov 23, 2000·Accounts of Chemical Research·R B HillW F DeGrado
Jul 17, 1998·Nature Structural Biology·H W Hellinga
Jun 29, 2000·Annual Review of Biochemistry·W F DeGradoA Lombardi
Apr 13, 2012·PLoS Computational Biology·J Dongun KimPaul G Falkowski
Mar 26, 2014·Chemical Reviews·Fangting YuVincent L Pecoraro
Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·M A DwyerH W Hellinga
May 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Christopher Horst LilligArne Holmgren
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·D E BensonH W Hellinga
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·A LombardiW F DeGrado
May 14, 2014·Annual Review of Analytical Chemistry·Muhammad Safwan AkramElizabeth A H Hall
Nov 5, 1999·Journal of Molecular Biology·P Koehl, M Levitt
Dec 26, 2015·Journal of the American Chemical Society·Clément AussignarguesCheryl A Kerfeld
Oct 10, 2015·Biochimica Et Biophysica Acta·Vikas NandaDror Noy
May 28, 2009·Protein Science : a Publication of the Protein Society·James J Havranek, David Baker
May 30, 2006·Protein Science : a Publication of the Protein Society·Matthieu NouaillerFrançoise Guerlesquin
Mar 21, 1998·Folding & Design·H W Hellinga
Sep 8, 2001·Journal of Molecular Biology·M López de la PazL Serrano
Feb 14, 2017·Organic & Biomolecular Chemistry·Andreas SchrimpfArmin Geyer
Mar 5, 2019·Chemical Communications : Chem Comm·Sara H MejiasAitziber L Cortajarena
Nov 28, 2013·Biopolymers·Marina FaiellaGiovanna Ghirlanda
Sep 6, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Amanda GalambasEric M Shepard
Oct 27, 1997·Science·W F DeGrado
Sep 26, 2007·The Journal of Biological Chemistry·Brian E WeinerWalter J Chazin
Aug 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying-Wu Lin
Dec 5, 2006·Inorganic Chemistry·Kinesha L HarrisSonya J Franklin
Nov 17, 2001·Chemical Reviews·D A Moffet, M H Hecht

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.