The RCAS-TVA system for introduction of oncogenes into selected somatic mammary epithelial cells in vivo.

Journal of Mammary Gland Biology and Neoplasia
Jay P Reddy, Yi Li

Abstract

We have reported the use of the RCAS-TVA system to model sporadic tumorigenesis upon oncogenic activation in somatic mammary epithelial cells in the mouse. Here we review the advantages of this approach as compared to conventional mouse models with transgenic oncogene expression. We also in detail describe the RCAS-TVA method for introducing genes into somatic mammary epithelial cells engineered to express the avian receptor tva. This method may be particularly useful in modeling oncogenic activation and subsequent tumorigenesis in distinct breast epithelial cell sub-populations, including progenitor cells.

References

Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M J FederspielS H Hughes
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·E C Holland, H E Varmus
Apr 17, 2001·Experimental Cell Research·H KimD N Foster
Feb 3, 2005·Molecular and Cellular Biology·Brian C LewisHarold E Varmus
Nov 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zhijun DuYi Li
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jennifer P MortonBrian C Lewis
Nov 21, 2007·Transgenic Research·Virginie S VervoortElena B Pasquale
Jun 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Justin SausvilleRicardo A Feldman
Jul 2, 2008·Neoplasia : an International Journal for Oncology Research·Stefan K SiwkoYi Li
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Barbara SeidlerDieter Saur

❮ Previous
Next ❯

Citations

Jun 18, 2011·Journal of Mammary Gland Biology and Neoplasia·Jay P Reddy, Yi Li
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jay P ReddyYi Li
Oct 15, 2013·Journal of Visualized Experiments : JoVE·Silva KrauseDonald E Ingber

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.