PMID: 8609158Apr 1, 1996Paper

The rDNA transcription machinery is assembled during mitosis in active NORs and absent in inactive NORs

The Journal of Cell Biology
P RousselD Hernandez-Verdun

Abstract

In cycling cells, the rDNAs are expressed from telophase to the end of G2 phase. The early resumption of rDNA transcription at telophase raises the question of the fate of the rDNA transcription machinery during mitosis. At the beginning of mitosis, rDNA transcription is arrested, and the rDNAs are clustered in specific chromosomal sites, the nucleolar organizer regions (NOR). In human cells, we demonstrate that the rDNA transcription machinery, as defined in vitro, is colocalized in some NORs and absent from others whatever the mitotic phase: RNA polymerase I and the RNA polymerase I transcription factors, upstream binding factor and promoter selectivity factor (as verified for TATA-binding protein and TATA-binding protein-associated factor for RNA polymerase I [110]), were colocalized in the same NORs. The RNA polymerase I complex was localized using two different antibodies recognizing the two largest subunits or only the third largest subunit, respectively. These two antibodies immunoprecipitated the RNA polymerase I complex in interphase cells as well as in mitotic cells. These results clearly indicated that the RNA polymerase I complex remained assembled during mitosis. In addition, RNA polymerase I and the transcription ...Continue Reading

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Citations

Jun 26, 2001·Molecular Reproduction and Development·V BaranJ E Flechon
Aug 1, 1996·Histochemistry and Cell Biology·M F TrendelenburgH Spring
Oct 18, 2008·Chromosoma·Peter C McKeown, Peter J Shaw
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