The reaction of Escherichia coli cytochrome bo with H2O2: evidence for the formation of an oxyferryl species by two distinct routes

FEBS Letters
T BrittainN J Watmough

Abstract

We have re-examined the reaction of fast oxidised cytochrome bo with H202 in a stopped-flow spectrophotometer. Monitoring the reaction at 582 nm allows us to observe the formation and decay of a spectroscopically distinct intermediate which accumulates transiently prior to the formation of an oxyferryl species previously characterised in this laboratory (Watmough, N.J., Cheesman, M.R., Greenwood, C. and Thomson, A.J. (1994) Biochem. J. 300, 469-475 [1]). The reaction shows three distinct phases of which the fast and intermediate phases are bimolecular and show a marked pH dependence. Initially these results appeared incompatible with the report that only one equivalent of H202 is required to generate the oxyferryl species (Moody, A.J. and Rich, P.R. (1994) Eur. J. Biochem. 226, 731-737 [21]. However, these data can be reconciled by a branched reaction mechanism whose contributions differ according to the peroxide concentration used.

References

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Citations

Aug 6, 2002·Biochimica Et Biophysica Acta·Peter R RichPeter Heathcote
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Iris von der HochtHartmut Michel
Jul 14, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Vasily S OganesyanAndrew J Thomson
Jan 21, 2015·Chemical Reviews·Shinya Yoshikawa, Atsuhiro Shimada
May 21, 2014·Biochemistry·Daniel JancuraMarian Fabian
Apr 22, 2004·Biochimica Et Biophysica Acta·Olöf Einarsdóttir, Istvan Szundi
Mar 12, 2021·Biochemistry. Biokhimii︠a︡·Ludmila MikulovaMarian Fabian
Dec 28, 1999·Biochimica Et Biophysica Acta·S JünemannP R Rich
May 17, 2000·Biochimica Et Biophysica Acta·D Zaslavsky, R B Gennis
Apr 22, 2004·Biochimica Et Biophysica Acta·Marian FabianGraham Palmer
Apr 22, 2004·Biochimica Et Biophysica Acta·Robert S Pitcher, Nicholas J Watmough
Nov 15, 2000·Biochemistry·A JasaitisM I Verkhovsky

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