The reaction of NADPH with bovine mitochondrial NADH:ubiquinone oxidoreductase revisited: I. Proposed consequences for electron transfer in the enzyme.

Journal of Bioenergetics and Biomembranes
S P Albracht

Abstract

Bovine NADH:ubiquinone oxidoreductase (Complex I) is the first complex in the mitochondrial respiratory chain. It has long been assumed that it contained only one FMN group. However, as demonstrated in 2003, the intact enzyme contains two FMN groups. The second FMN was proposed to be located in a conserved flavodoxin fold predicted to be present in the PSST subunit. The long-known reaction of Complex I with NADPH differs in many aspects from that with NADH. It was proposed that the second flavin group was specifically involved in the reaction with NADPH. The X-ray structure of the hydrophilic domain of Complex I from Thermus thermophilus (Sazanov and Hinchliffe 2006, Science 311, 1430-1436) disclosed the positions of all redox groups of that enzyme and of the subunits holding them. The PSST subunit indeed contains the predicted flavodoxin fold although it did not contain FMN. Inspired by this structure, the present paper describes a re-evaluation of the enigmatic reactions of the bovine enzyme with NADPH. Published data, as well as new freeze-quench kinetic data presented here, are incompatible with the general opinion that NADPH and NADH react at the same site. Instead, it is proposed that these pyridine nucleotides react at o...Continue Reading

References

Feb 1, 1979·Archives of Biochemistry and Biophysics·Y M Galante, Y Hatefi
Jul 9, 1976·Biochimica Et Biophysica Acta·G Dooijewaard, E C Slater
Feb 7, 1977·Biochimica Et Biophysica Acta·S P AlbrachtB V Swol
Oct 12, 1977·Biochimica Et Biophysica Acta·S P Albracht, J Subramanian
Jan 1, 1979·Methods in Enzymology·T P Singer
Nov 1, 1992·European Journal of Biochemistry·R van BelzenS P Albracht
Aug 1, 1992·Quarterly Reviews of Biophysics·J E Walker
Aug 30, 1990·Biochimica Et Biophysica Acta·A B Kotlyar, A D Vinogradov
May 30, 1989·Biochimica Et Biophysica Acta·R van Belzen, S P Albracht
Feb 20, 1973·Biochemical and Biophysical Research Communications·Y Hatefi
Feb 8, 1967·Biochemical and Biophysical Research Communications·Y Hatefi, K E Stempel
Mar 11, 1968·Archives of Biochemistry and Biophysics·G LenazK Kfolkers
Jun 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·Y Hatefi
Mar 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·M GutmanJ E Casida
Apr 6, 1966·Biochemical and Biophysical Research Communications·R B BeecheyA M Roberton
Mar 14, 1980·Biochimica Et Biophysica Acta·S P Albracht
Dec 31, 1982·Biochimica Et Biophysica Acta·H Beinert, S P Albracht
Feb 16, 1995·Nature·A VolbedaJ C Fontecilla-Camps
Jun 15, 1994·European Journal of Biochemistry·A M De Jong, S P Albracht

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.