Jan 1, 1977

The reactive behaviour of the cell nucleus after a pretreatment with a bromide solution and the application of the calcium-cobalt-method for the ATPase evidence (author's transl)

Acta histochemica
H Hettwer, H G Goslar

Abstract

A selective staining of the nucleus of both the cells in an islet (of Langerhans) and the acinar cells could be observed after treatment with bromide-water in an ATPase-medium (calcium-cobalt-method) on a cryostatsection of rat pancreas. Furthermore the behaviour of the other metal ion forming a complex has been tested in place of cobalt salt. As conditioned for the staining reaction the hydrolysis of nucleic acid with oxidation of its base by bromide-water and also the complex formation of Co++ with compounds similar to alloxan, resulted from oxidation were discussed.

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Mentioned in this Paper

Calcium [EPC]
Bromides
Calcium
Adenosine Triphosphatases
Cell Nucleus
Oxidation
Bromides Measurement
Rat Pancreas
Staining and Labeling
Alloxan

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