The readthrough region of Potato mop-top virus (PMTV) coat protein encoding RNA, the second largest RNA of PMTV genome, undergoes structural changes in naturally infected and experimentally inoculated plants

Archives of Virology
M SandgrenJ P T Valkonen

Abstract

Molecular data on Potato mop-top virus (PMTV), genus Pomovirus, is currently mostly based on analysis of two Scottish isolates, PMTV-S and PMTV-T. Here we report the complete sequence of "the coat protein (CP) encoding RNA" of an isolate of PMTV obtained from the field in Sweden. Our data show that this RNA (3134 nt) is the second largest of the three RNA species in the tripartite PMTV genome, and it should, therefore, be referred to as RNA 2. This nomenclature is consistent with other pomoviruses. The sequence of the readthrough domain (RT) of RNA 2 was determined also in two additional field isolates of PMTV from Finland and Denmark. All three isolates contained a novel, 109 nucleotides long sequence at the 3'-end of the RT, which has not been found in PMTV-S and PMTV-T. Hence, our data suggest that the RNA 2 sequences previously described for the isolates PMTV-T and PMTV-S may represent deletion derivatives. The C-proximal half of RT contained many amino acid (aa) differences among the isolates, in contrast to only few aa differences in the N-proximal part of RT. Deletion variants of RNA 2 were generated from the Nordic isolates in potato tubers infected in the field, and in the mechanically inoculated test plants. All delet...Continue Reading

Citations

Jul 31, 2004·Annual Review of Phytopathology·D'Ann RochonRon Reade
Jan 15, 2014·Journal of Experimental Botany·Andrey G Solovyev, Eugene I Savenkov
Mar 11, 2015·Archives of Virology·Ulrike BeuchAnders Kvarnheden
Oct 29, 2013·Journal of Virological Methods·Muhammad ArifMuhammad Fahim
Mar 11, 2009·Molecular Plant-microbe Interactions : MPMI·Lesley TorranceEugene I Savenkov
Sep 28, 2005·The Journal of General Virology·N I LukhovitskayaE I Savenkov

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