The real-time path of translation factor IF3 onto and off the ribosome

Molecular Cell
Attilio FabbrettiClaudio O Gualerzi


Translation initiation factor IF3 is an essential bacterial protein, consisting of two domains (IF3C and IF3N) separated by a linker, which interferes with ribosomal subunit association, promotes codon-anticodon interaction in the P site, and ensures translation initiation fidelity. Using time-resolved chemical probing, we followed the dynamic binding path of IF3 on the 30S subunit and its release upon 30S-50S association. During binding, IF3 first contacts the platform (near G700) of the 30S subunit with the C domain and then the P-decoding region (near A790) with its N domain. At equilibrium, attained within less than a second, both sites are protected, but before reaching binding equilibrium, IF3 causes additional transient perturbations of both the platform edge and the solvent side of the subunit. Upon 30S-50S association, IF3 dissociates concomitantly with the establishment of the 30S-50S bridges, following the reverse path of its binding with the IF3N-A790 interaction being lost before the IF3C-G700 interaction.


Feb 24, 1976·Biochemistry·C L Pon, Claudio O Gualerzi
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W E TapprichAlbert E Dahlberg
May 1, 1988·Journal of General Microbiology·M E ElishC F Earhart
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C L Pon, Claudio O Gualerzi
May 25, 1993·Nucleic Acids Research·P A KingM Brenowitz
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Anna La TeanaClaudio O Gualerzi
Jul 22, 1996·FEBS Letters·M GötteH Heumann
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P McCutcheonJ Frank
Aug 10, 2000·The Journal of Cell Biology·Rajendra K AgrawalJ Frank
May 8, 2001·Science·J M OgleV Ramakrishnan
Aug 14, 2001·The EMBO Journal·Dezemona PetrelliClaudio O Gualerzi
Oct 31, 2001·Molecular Cell·A Dallas, Harry F Noller
Mar 23, 2002·Cell·V Ramakrishnan
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·Claudio O GualerziC L Pon
Jul 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Antón Vila-SanjurjoJamie H Doudna Cate
Aug 6, 2003·Journal of Molecular Biology·Dezemona PetrelliAnna La Teana
Feb 17, 2005·Journal of Molecular Biology·Scott P HennellyWalter E Hill
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Nov 26, 2005·Molecular Cell·Laura Lancaster, Harry F Noller
Dec 20, 2005·The EMBO Journal·Ivan B LomakinTatyana V Pestova
May 26, 2006·The EMBO Journal·Ayman AntounMåns Ehrenberg
Sep 9, 2006·Science·Maria SelmerV Ramakrishnan


Dec 13, 2007·Quarterly Reviews of Biophysics·Christopher S Fraser, Jennifer A Doudna
Feb 26, 2009·Biochemistry·Brooke E Christian, Linda L Spremulli
Oct 20, 2007·Nature Protocols·Pilar TijerinaRick Russell
Apr 16, 2013·Nature Structural & Molecular Biology·Margaret M Elvekrog, Ruben L Gonzalez
Apr 21, 2012·Critical Reviews in Biochemistry and Molecular Biology·Pohl Milón, Marina V Rodnina
Dec 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Clarence Ling, Dmitri N Ermolenko
Jun 6, 2009·Current Opinion in Structural Biology·Alexander G MyasnikovBruno P Klaholz
Feb 27, 2009·Current Opinion in Cell Biology·Marina V Rodnina, Wolfgang Wintermeyer
Sep 5, 2015·Nucleic Acids Research·Akanksha GoyalMarina V Rodnina
Jan 8, 2008·Journal of Molecular Biology·Ashesh A SaraiyaPhilip R Cunningham
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tanvir R ShaikhRajendra K Agrawal
Aug 12, 2015·Cellular and Molecular Life Sciences : CMLS·Claudio O Gualerzi, Cynthia L Pon
Apr 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Attilio FabbrettiPaola Fucini
Apr 16, 2008·Expert Opinion on Therapeutic Targets·Letizia BrandiClaudio O Gualerzi
Sep 18, 2007·Journal of Molecular Biology·Christina GrigoriadouBarry S Cooperman
Oct 22, 2008·Journal of Molecular Biology·Md Emdadul Haque, Linda L Spremulli
Apr 18, 2018·Cold Spring Harbor Perspectives in Biology·Marina V Rodnina
Dec 18, 2008·Chembiochem : a European Journal of Chemical Biology·Ella Zimmerman, Ada Yonath
Mar 2, 2021·Frontiers in Microbiology·Elena M MaksimovaAndrey L Konevega

Related Concepts

Alkalescens-Dispar Group
DNA Conformation
Proteins, Recombinant DNA
RNA, Bacterial
RNA, Ribosomal, 16S
Tertiary Protein Structure
Escherichia coli Proteins
Prokaryotic Initiation Factor-3

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.