The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction

Molecular Microbiology
B MartinJ P Claverys

Abstract

The recently identified recA gene of the naturally transformable bacterium Streptococcus pneumoniae has been further characterized by constructing a recA null mutation and by investigating its regulation. The recA mutation has been shown to confer both DNA repair (as judged from sensitivity to u.v. and methyl methane sulphonate) and recombination deficiencies. Plasmid transformation into the recA mutant was also drastically reduced. Western blotting established that recA gene expression is increased several fold at the onset of competence for genetic transformation. Increased expression was associated with the appearance of a recA-specific transcript, approximately 5.7 kb long. This transcript indicated that recA is part of a competence-inducible (cin) operon. The major (about 4.3 kb) transcript detected from non-competent cells did not include cinA, the first gene in the operon, suggesting that this gene could be specifically required at some stage in the transformation process. Detection of small amounts of the 5.7 kb polycistronic mRNA in cells treated with mitomycin C suggested that the operon could also be damage inducible. In addition, mitomycin C treatment of a recA- lysogenic strain did not lead to prophage induction an...Continue Reading

References

Aug 1, 1976·Journal of Virology·R D Porter, W R Guild
Dec 11, 1992·Nucleic Acids Research·B MartinJ P Claverys
May 1, 1992·Journal of Bacteriology·A Raymond-Denise, N Guillen
Jul 1, 1990·Molecular & General Genetics : MGG·P CeglowskiJ C Alonso
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·A I Roca, M M Cox
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A Tomasz, J L Mosser
Jan 1, 1971·Molecular & General Genetics : MGG·W J Harris, G C Barr
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J P ClaverysA M Sicard
Oct 1, 1982·Journal of Bacteriology·C C MuckermanS A Lacks
Jan 1, 1981·Molecular & General Genetics : MGG·C W Saunders, W R Guild
Jan 1, 1982·Molecular & General Genetics : MGG·J L Raina, F L Macrina
Mar 1, 1980·Journal of Bacteriology·D J Moynet, G J Tiraby
Apr 1, 1980·Mutation Research·A M GascA M Sicard
Mar 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·A TOMASZ, R D HOTCHKISS
Jan 1, 1928·The Journal of Hygiene·F Griffith

❮ Previous
Next ❯

Citations

Nov 9, 2000·Archives of Biochemistry and Biophysics·S E Steffen, F R Bryant
Mar 27, 1998·Molecular Microbiology·H R MasureB Spellerberg
May 22, 2001·Molecular Microbiology·G W LauD W Holden
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marc PrudhommeJean-Pierre Claverys
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·O HumbertJ P Claverys
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·F Pasta, M A Sicard
Jul 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·P GarcíaR López
Oct 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·I Mortier-BarriereJ P Claverys
Jan 26, 2000·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M C Trombe
May 26, 2009·Journal of Bacteriology·Andrew PiotrowskiDonald A Morrison
Jul 3, 2007·Journal of Bacteriology·Donald A MorrisonJean-Pierre Claverys
Dec 26, 2001·Journal of Bacteriology·Ange-Patricia Tchamedeu KameniMathieu Picardeau
Aug 5, 2003·Journal of Bacteriology·Pablo CastánJosé Berenguer
Jun 15, 2006·Annual Review of Microbiology·Jean-Pierre ClaverysBernard Martin
Dec 18, 2013·PloS One·Guiomar Sánchez-CarrónÁlvaro Sánchez-Ferrer
Aug 16, 2011·Journal of Visualized Experiments : JoVE·Imke G de JongJan-Willem Veening
May 29, 2013·Extremophiles : Life Under Extreme Conditions·Marleen van WolferenSonja-Verena Albers
Mar 12, 2013·TheScientificWorldJournal·Satoko InagakiMichiyo Matsumoto-Nakano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.