The recent escalation in strength of pyrethroid resistance in Anopheles coluzzi in West Africa is linked to increased expression of multiple gene families

BMC Genomics
Kobié H ToéChristopher M Jones

Abstract

Since 2011, the level of pyrethroid resistance in the major malaria mosquito, Anopheles coluzzi, has increased to such an extent in Burkina Faso that none of the long lasting insecticide treated nets (LLINs) currently in use throughout the country kill the local mosquito vectors. We investigated whether this observed increase was associated with transcriptional changes in field-caught Anopheles coluzzi using two independent whole-genome microarray studies, performed in 2011 and 2012. Mosquitoes were collected from south-west Burkina Faso in 2011 and 2012 and insecticide exposed or non-exposed insects were compared to laboratory susceptible colonies using whole-genome microarrays. Using a stringent filtering process we identified 136 genes, including the well-studied detoxification enzymes (p450 monoxygenases and esterases) and non-detoxification genes (e.g. cell transporters and cuticular components), associated with pyrethroid resistance, whose basal expression level increased during the timeframe of the study. A subset of these were validated by qPCR using samples from two study sites, collected over 3 years and marked increases in expression were observed each year. We hypothesise that these genes are contributing to this ra...Continue Reading

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Citations

Nov 10, 2015·Insect Biochemistry and Molecular Biology·Sulaiman S IbrahimCharles S Wondji
Aug 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Chris Bass, Christopher M Jones
Aug 16, 2016·Malaria Journal·Bart G J KnolsMatthew B Thomas
Jul 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Vasileia BalabanidouJohn Vontas
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Dec 18, 2018·Frontiers in Immunology·Augustina FrimpongWilfred Ndifon
May 17, 2017·Cold Spring Harbor Perspectives in Medicine·Hilary Ranson
Sep 12, 2020·Parasites & Vectors·Eunice Oluwatobiloba AdedejiEzekiel Adebiyi
Jan 9, 2021·Malaria Journal·Jeff JonesPhilip J McCall
Apr 9, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jianhai YinNing Xiao
Jul 28, 2021·Communications Biology·Kelsey L AdamsFlaminia Catteruccia

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Datasets Mentioned

BETA
E-MTAB-3212
E-MTAB-2859
E-MTAB-2875

Methods Mentioned

BETA
PCR
genotyping
chip
feature extraction
reverse-transcription quantitative PCR

Software Mentioned

R
LIMMA
ArrayExpress
Haploview
PrimerBLAST

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