The receptor for activated C-kinase-I (RACK-I) anchors activated PKC-beta on melanosomes

Journal of Cell Science
Hee-Young ParkB A Gilchrest

Abstract

Protein kinase C (PKC), a family of at least eleven isoforms, mediates numerous cell functions. In human melanocytes, alpha, beta, delta, epsilon and zeta isoforms of PKC are expressed, but uniquely PKC-beta activates tyrosinase, the key and the rate-limiting enzyme in melanogenesis, by phosphorylating specific serine residues on its cytoplasmic domain. To investigate the mechanism by which only PKC-beta phosphorylates tyrosinase, we examined the expression of receptor for activated C-kinase-I (RACK-I), a receptor specific for activated PKC-beta, on the surface of melanosomes, the specialized organelle in which melanogenesis occurs. Immunoblot analysis of purified melanosomes revealed that RACK-I is readily detectable. Immunoprecipitation of RACK-I from purified melanosomes, followed by immunoblot analysis using antibody against PKC-beta, revealed abundant PKC-beta, whereas PKC-alpha was not detected when immunoblot analysis was performed using antibody against PKC-alpha. Activation of PKC in melanocytes increased the level of PKC-beta co-immunoprecipitated with RACK-I, while the level of melanosome-associated RACK-I decreased when melanocytes were treated chronically with the 12-0-tetradecanoyl-phorbol 13-Acetate (TPA), a cond...Continue Reading

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Citations

Mar 2, 2006·Molecular and Cellular Biology·Alejandra CollazosDominique Joubert
Feb 5, 2010·International Journal of Cell Biology·Hee-Young ParkBarbara A Gilchrest
Dec 14, 2011·The Journal of Investigative Dermatology·Mina Yaar, Hee-Young Park
Aug 17, 2012·The Journal of Investigative Dermatology·Masakazu KawaguchiVincent J Hearing
Apr 22, 2009·Experimental Dermatology·Paul L BigliardiMei Bigliardi-Qi
Jan 30, 2014·Pigment Cell & Melanoma Research·Hyejung JungEok-Soo Oh
Sep 27, 2005·Biochemical and Biophysical Research Communications·Choonshik ShinYoongho Lim
Apr 14, 2016·The Journal of Investigative Dermatology·Soujiro MarubashiMitsunori Fukuda
Dec 18, 2009·Pigment Cell & Melanoma Research·John P VorisMitchell F Denning
May 15, 2012·Pigment Cell & Melanoma Research·Mitchell F Denning
Jul 30, 2014·Archives of Pharmacal Research·Seung Deok HongYoungsoo Kim
Mar 1, 2019·International Journal of Molecular Sciences·Jung-Im NaKyung-Chan Park
Feb 13, 2021·Biochemical Pharmacology·Christian Bailly
Aug 8, 2021·International Journal of Molecular Sciences·Ilandarage Menu Neelaka MolagodaGi-Young Kim

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