The receptor-like cytoplasmic kinase BSR1 mediates chitin-induced defense signaling in rice cells

Bioscience, Biotechnology, and Biochemistry
Yasukazu KandaMasaki Mori

Abstract

Broad-Spectrum Resistance 1 (BSR1) encodes a rice receptor-like cytoplasmic kinase, and enhances disease resistance when overexpressed. Rice plants overexpressing BSR1 are highly resistant to diverse pathogens, including rice blast fungus. However, the mechanism responsible for this resistance has not been fully characterized. To analyze the BSR1 function, BSR1-knockout (BSR1-KO) plants were generated using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Experiments using suspension-cultured cells revealed that defense responses including H2O2 production (i.e. oxidative burst) and expression of defense-related genes induced by autoclaved conidia of the rice blast fungus significantly decreased in BSR1-KO cells. Furthermore, a treatment with chitin oligomers which function as microbe-associated molecular patterns (MAMPs) of the rice blast fungus resulted in considerably suppressed defense responses in BSR1-KO cells. These results suggest that BSR1 is important for the rice innate immunity triggered by the perception of chitin.

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Citations

May 3, 2018·Annual Review of Plant Biology·Xiangxiu Liang, Jian-Min Zhou
Aug 15, 2017·Plant Signaling & Behavior·Tsutomu KawasakiKoji Yamaguchi
Mar 18, 2020·Critical Reviews in Biotechnology·Andrey S MarchevMilen I Georgiev
Jul 5, 2019·International Journal of Molecular Sciences·Yue ZhangLiyong Cao
Aug 6, 2020·International Journal of Molecular Sciences·Yasukazu KandaMasaki Mori
Jul 19, 2019·Molecular Biology Reports·Farhad Nazarian-FirouzabadiAjjamada C Kushalappa
Sep 8, 2017·Frontiers in Plant Science·Muhammad N SattarSuliman A Al-Khateeb
Aug 18, 2021·Molecular Cell·Thomas A DeFalco, Cyril Zipfel

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Methods Mentioned

BETA
transgenic
PCR
targeted mutation

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