The receptor tyrosine kinase Ror2 associates with and is activated by casein kinase Iepsilon

The Journal of Biological Chemistry
Shuichi KaniYasuhiro Minami

Abstract

Ror2, a member of the mammalian Ror family of receptor tyrosine kinases, plays important roles in developmental morphogenesis, although the mechanism underlying activation of Ror2 remains largely elusive. We show that when expressed in mammalian cells, Ror2 associates with casein kinase Iepsilon (CKIepsilon), a crucial regulator of Wnt signaling. This association occurs primarily via the cytoplasmic C-terminal proline-rich domain of Ror2. We also show that Ror2 is phosphorylated by CKIepsilon on serine/threonine residues, in its C-terminal serine/threonine-rich 2 domain, resulting in autophosphorylation of Ror2 on tyrosine residues. Furthermore, it was found that association of Ror2 with CKIepsilon is required for its serine/threonine phosphorylation by CKIepsilon. Site-directed mutagenesis of tyrosine residues in Ror2 reveals that the sites of phosphorylation are contained among the five tyrosine residues in the proline-rich domain but not among the four tyrosine residues in the tyrosine kinase domain. Moreover, we show that in mammalian cells, CKIepsilon-mediated phosphorylation of Ror2 on serine/threonine and tyrosine residues is followed by the tyrosine phosphorylation of G protein-coupled receptor kinase 2, a kinase with a...Continue Reading

References

May 9, 1988·FEBS Letters·J GrandeE Itarte
Jan 1, 1987·Diabetes·R A Roth, J Beaudoin
Apr 18, 1997·The Journal of Biological Chemistry·M E CunninghamL A Greene
Jun 25, 1998·Current Biology : CB·P Masiakowski, G D Yancopoulos
Oct 6, 1998·Annual Review of Biochemistry·J A PitcherR J Lefkowitz
May 13, 1999·The Journal of Biological Chemistry·A J BarbierT B Patel
Oct 12, 1999·Nature·J M PetersJ M Graff
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·C SakanakaL T Williams
Jan 29, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S TakeuchiY Minami
Jun 20, 2000·Trends in Genetics : TIG·M KühlR T Moon
Oct 25, 2000·Mechanisms of Development·M SeftonM A Nieto
Nov 1, 2000·Cell·J Schlessinger
Jun 27, 2001·The Journal of Biological Chemistry·M KishidaA Kikuchi
Jul 17, 2001·Development Genes and Evolution·R Al-ShawiJ P Simons
Aug 29, 2001·The Journal of Cell Biology·E LeeM W Kirschner
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhong-Hua GaoDavid M Virshup
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·W C Forrester
May 16, 2002·Journal of Medical Genetics·M A Patton, A R Afzal
Jul 13, 2002·Trends in Biochemical Sciences·Yanning Ding, Trevor Dale
May 10, 2003·The Journal of Biological Chemistry·Ana ElorzaFederico Mayor
Jul 4, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Isao OishiYasuhiro Minami
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Georg C SchwabeStefan Mundlos

❮ Previous
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Citations

Nov 16, 2012·Nature Reviews. Molecular Cell Biology·Christof Niehrs
Sep 2, 2009·The Journal of Biological Chemistry·Amanda MikelsRoel Nusse
Jan 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hans A Kestler, Michael Kühl
Aug 28, 2009·Pigment Cell & Melanoma Research·Michael P O'Connell, Ashani T Weeraratna
May 12, 2010·Molecular and Cellular Biology·Michiru NishitaYasuhiro Minami
Mar 30, 2012·Neuro-Signals·Charlotte E J ClarkHelen M Cooper
Aug 31, 2013·Molecular Neurobiology·Iveta M PetrovaJasprina N Noordermeer
Nov 15, 2006·The Journal of Cell Biology·Michiru NishitaYasuhiro Minami
Apr 4, 2015·Expert Opinion on Therapeutic Targets·Mahdi ShabaniFazel Shokri
Jan 30, 2015·Cell Communication and Signaling : CCS·Markéta KauckáVitezslav Bryja
Jun 7, 2014·Frontiers in Oncology·Uwe KnippschildJoachim Bischof
Oct 14, 2008·Trends in Cell Biology·Jennifer L GreenPaul W Sternberg
Sep 10, 2011·Acta Biochimica Et Biophysica Sinica·Antara De
May 5, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mitsuharu Endo, Yasuhiro Minami
Feb 4, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Xiaoyuan QiYasuhiro Minami
Feb 22, 2018·Molecular Oncology·Josué CurtoMireia Duñach
Nov 13, 2015·Journal of Virology·Wendy J van ZuylenWilliam D Rawlinson
Jun 4, 2014·Molecular and Cellular Biology·Michiru NishitaYasuhiro Minami
Dec 19, 2015·PloS One·Martina PodleschnyAnnette Borchers
Jun 17, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yasuhiro MinamiMichiru Nishita
Jan 12, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Makiko YamadaHiroki Otani
Dec 13, 2012·International Journal of Cancer. Journal International Du Cancer·Caroline E FordRobyn L Ward
Aug 31, 2017·Science Signaling·Shunsuke UeharaYasuhiro Kobayashi
Mar 11, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Florian WitteSigmar Stricker
Dec 1, 2011·The Journal of Biological Chemistry·Kaoru YamagataYasuhiro Minami
Jul 19, 2012·IUBMB Life·Hyun-Jung ChoiYoung-Guen Kwon
Aug 19, 2020·Scientific Reports·Dongli LiuCaroline E Ford
Nov 8, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroyuki YamamotoYasuhiro Minami
Jun 13, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Xin LiYasuhiro Minami
Jan 28, 2017·Scientific Reports·Michiru NishitaYasuhiro Minami
Feb 24, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Dayong RenMichiru Nishita
Jan 16, 2021·Cells·Kerstin MenckAnnalen Bleckmann
Jan 27, 2021·Cancers·Dongli LiuCaroline E Ford
Dec 3, 2020·International Journal of Molecular Sciences·Pavlína JanovskáVítězslav Bryja

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