The RecJ DNase strongly suppresses genomic integration of short but not long foreign DNA fragments by homology-facilitated illegitimate recombination during transformation of Acinetobacter baylyi

Molecular Microbiology
Klaus HarmsWilfried Wackernagel

Abstract

Homology-facilitated illegitimate recombination (HFIR) promotes genomic integration of foreign DNA with a single segment homologous to the recipient genome by homologous recombination in the segment accompanied by illegitimate fusion of the heterologous sequence. During natural transformation of Acinetobacter baylyi HFIR occurs at about 0.01% of the frequency of fully homologous recombination. The role of the 5' single-strand-specific exonuclease RecJ in HFIR was investigated. Deletion of recJ increased HFIR frequency about 20-fold compared with wild type while homologous recombination was not affected. Illegitimate fusion sites were predominantly located within 360 nucleotides away from the homology whereas in wild type most fusion sites were distal (500-2500 nucleotides away). RecJ overproduction reduced the HFIR frequency to half compared with wild type, and transformants with short foreign DNA segments were diminished, leading to on average 866 foreign nucleotides integrated per event (682 in wild type, 115 in recJ). In recJ always the 3' ends of donor DNA were integrated at the homology whereas in wild type these were 3' or 5'. RecJ apparently suppresses HFIR by degrading 5' non-homologous DNA tails at the post-synaptic st...Continue Reading

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Citations

Mar 2, 2010·Applied Microbiology and Biotechnology·Matthias Brigulla, Wilfried Wackernagel
Dec 12, 2012·Journal of Bacteriology·Jialiang XuBiao Kan
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Søren Overballe-PetersenEske Willerslev
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Sep 14, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ole Herman AmburDaniel E Rozen
Mar 25, 2014·Applied and Environmental Microbiology·Shweta FulsundarKaare M Nielsen
Sep 2, 2020·Nucleic Acids Research·Kaiying ChengYuejin Hua
Apr 20, 2016·Cold Spring Harbor Perspectives in Biology·William P Hanage
Feb 17, 2017·BMC Microbiology·Nils HülterP J Johnsen
Dec 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Klaus HarmsP J Johnsen

Related Concepts

RecJ protein, Bacteria
Mima
Bacterial Proteins
DNA, Bacterial
Exodeoxyribonuclease
Heteroduplex DNA
Episomes
Recombination, Genetic
Transformation, Bacterial
Nested Polymerase Chain Reaction

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