The redox chemistry of the covalently immobilized native and low-pH forms of yeast iso-1-cytochrome c

Journal of the American Chemical Society
Carlo Augusto BortolottiMarco Sola

Abstract

Cyclic voltammetry experiments were carried out on native Saccharomyces cerevisiae iso-1-cytochrome c and its C102T/N62C variant immobilized on bare polycrystalline gold electrode through the S-Au bond formed by a surface cysteine. Experiments were carried out at different temperatures (5-65 degrees C) and pH values (1.5-7). The E degrees ' value at pH 7 (+370 mV vs SHE) is approximately 100 mV higher than that for the protein in solution. This difference is enthalpic in origin and is proposed to be the result of the electrostatic repulsion among the densely packed molecules onto the electrode surface. Two additional electrochemical waves are observed upon lowering the pH below 5 (E degrees ' = +182 mV) and 3 (E degrees ' = +71 mV), which are attributed to two conformers (referred to as "intermediate" and "acidic", respectively) featuring an altered heme axial ligation. This is the first determination of the reduction potential for low-pH conformers of cytochrome c in the absence of denaturants. Since the native form of cytochrome c can be restored, bringing back the pH to neutrality, the possibility offered by this transition to reversibly modulate the redox potential of cytochrome c is appealing for bioelectronic applications...Continue Reading

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Citations

Sep 13, 2011·Chemical Communications : Chem Comm·Claudio TavagnaccoMarco Borsari
Mar 23, 2012·Antioxidants & Redox Signaling·Shradha PrabhulkarChen-Zhong Li
May 1, 2014·The Journal of Physical Chemistry Letters·Isabella DaidoneCarlo Augusto Bortolotti
Apr 4, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Giulia Di RoccoMarco Sola
Jun 16, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Stefano MonariMarco Borsari
Jun 16, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gianantonio BattistuzziSachiko Yanagisawa
Jun 20, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gianantonio BattistuzziMarco Sola
Feb 12, 2014·Metallomics : Integrated Biometal Science·Antonio RanieriMarco Sola
Apr 1, 2016·Physical Chemistry Chemical Physics : PCCP·Laura Zanetti-Polzi, Stefano Corni
Sep 27, 2006·Physical Chemistry Chemical Physics : PCCP·Andrea AlessandriniPaolo Facci
Mar 25, 2011·Chemistry, an Asian Journal·Xiuwen WangYi-Tao Long
Mar 24, 2018·The Journal of Chemical Physics·Ivan Vyalov, Walter Rocchia
Jul 5, 2013·Physical Chemistry Chemical Physics : PCCP·Giulia Di RoccoMarco Sola
Jan 16, 2008·The Journal of Physical Chemistry. B·Stefano CasaliniMarco Sola
Nov 4, 2008·The Journal of Physical Chemistry. B·Jiu-Ju FengInez M Weidinger
Oct 16, 2008·Journal of the American Chemical Society·Stefano CasaliniMarco Sola
May 12, 2010·Journal of the American Chemical Society·Paolo PetrangoliniPaolo Facci
Jan 12, 2010·The Journal of Physical Chemistry. B·Stefano CasaliniMarco Sola
Sep 4, 2014·Journal of the American Chemical Society·Laura Zanetti-PolziStefano Corni
Jul 11, 2012·Biochemistry·Gianantonio BattistuzziMarco Sola
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonio RanieriGianantonio Battistuzzi
Mar 10, 2010·Archives of Biochemistry and Biophysics·Gianantonio BattistuzziMarco Sola

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