The redox-dependent regulation of satellite cells following aseptic muscle trauma (SpEED): study protocol for a randomized controlled trial

Trials
Konstantinos PapanikolaouIoannis G Fatouros

Abstract

Muscle satellite cells (SCs) are crucial for muscle regeneration following muscle trauma. Acute skeletal muscle damage results in inflammation and the production of reactive oxygen species (ROS) which may be implicated in SCs activation. Protection of these cells from oxidative damage is essential to ensure sufficient muscle regeneration. The aim of this study is to determine whether SCs activity under conditions of aseptic skeletal muscle trauma induced by exercise is redox-dependent. Based on the SCs content in their vastus lateralis skeletal muscle, participants will be classified as either high or low respondents. In a randomized, double-blind, crossover, repeated-measures design, participants will then receive either placebo or N-acetylcysteine (alters redox potential in muscle) during a preliminary 7-day loading phase, and for eight consecutive days following a single bout of intense muscle-damaging exercise. In both trials, blood samples and muscle biopsies will be collected, and muscle performance and soreness will be measured at baseline, pre-exercise, 2 and 8 days post exercise. Biological samples will be analyzed for redox status and SCs activity. Between trials, a 4-week washout period will be implemented. This stud...Continue Reading

Associated Clinical Trials

References

Oct 1, 1991·International Journal of Sports Medicine·M M Umnova, T P Seene
Feb 1, 1991·Clinical Pharmacokinetics·M R Holdiness
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 5, 2004·Histochemistry and Cell Biology·Fawzi KadiJan Henriksson
Jul 17, 2004·Free Radical Biology & Medicine·Wataru AoiToshikazu Yoshikawa
Dec 1, 2004·Medicine and Science in Sports and Exercise·Ioannis G FatourosGeorge Deliconstantinos
Jul 12, 2005·European Journal of Applied Physiology·Athanasios Z JamurtasK Nosaka
Jan 5, 2006·The Journal of Cell Biology·Shihuan KuangMichael A Rudnicki
Mar 31, 2007·Journal of Applied Physiology·Marcas M BammanJames M Cross
Nov 13, 2007·Current Opinion in Cell Biology·Fabien Le Grand, Michael A Rudnicki
Oct 21, 2009·Current Opinion in Clinical Nutrition and Metabolic Care·Rajavel ElangoPaul B Pencharz
Mar 12, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·James G Tidball, S Armando Villalta
Apr 1, 2010·Journal of Physiology and Biochemistry·A Zembron-LacnyK Szyszka
Apr 22, 2011·The American Journal of Clinical Nutrition·Anastasios A TheodorouAthanasios Z Jamurtas
May 12, 2011·International Journal of Sport Nutrition and Exercise Metabolism·Leonardo F FerreiraMichael B Reid
Jul 12, 2011·Respiratory Physiology & Neurobiology·Sarah D Corn, Thomas J Barstow
Nov 15, 2011·The Journal of Nutritional Biochemistry·Kathryn H MyburghCarine Smith
Nov 18, 2011·International Journal of Sport Nutrition and Exercise Metabolism·James N CobleyGraeme L Close
Jan 31, 2012·Cellular Signalling·Paul D RayYoshiaki Tsuji
Apr 25, 2012·Medicine and Science in Sports and Exercise·Leena ChoiMaciej S Buchowski
Jun 7, 2012·Methods in Molecular Biology·Craig Harris, Jason M Hansen
Jun 8, 2012·Journal of Muscle Research and Cell Motility·F Macaluso, K H Myburgh
Jan 9, 2013·Annals of Internal Medicine·An-Wen ChanDavid Moher
Jan 11, 2013·Physiological Reviews·Hang YinMichael A Rudnicki
Apr 27, 2013·Biochimica Et Biophysica Acta·Yuval SamuniMichael Berk
May 4, 2013·Critical Reviews in Food Science and Nutrition·J M Landete
Jul 9, 2013·Methods in Enzymology·Regina Brigelius-Flohé, Anna Patricia Kipp
Mar 1, 2014·Medicine and Science in Sports and Exercise·Katie May SlatteryAaron James Coutts
Apr 9, 2014·The Journal of Cell Biology·C Florian BentzingerMichael A Rudnicki
Sep 30, 2014·Scandinavian Journal of Medicine & Science in Sports·K KrügerF C Mooren
Mar 15, 2015·Biochimica Et Biophysica Acta·Fenglian RenCanhua Huang
Jun 5, 2015·PloS One·Dimitrios DraganidisIoannis G Fatouros
Sep 18, 2015·European Journal of Applied Physiology·Magni MohrIoannis G Fatouros
Dec 18, 2015·The American Journal of Clinical Nutrition·Russell J de SouzaLehana Thabane

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
biopsies
electron microscopy
X-ray
MDA
Protein Assay
electrophoresis

Software Mentioned

Image
ActiLife
Power
GE enCORE
Science Fit Diet 200 A
SPSS
Pro Plus

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.