The redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis

Proteomics
Bui Khanh ChiHaike Antelmann

Abstract

The MarR/DUF24-type repressor YodB controls the azoreductase AzoR1, the nitroreductase YodC and the redox-sensing regulator Spx in response to quinones and diamide in Bacillus subtilis. Previously, we showed using a yodBCys6-Ala mutant that the conserved Cys6 apparently contributes to the DNA-binding activity of YodB in vivo. Here, we present data that mutation of Cys6 to Ser led to a form of the protein that was reduced in redox-sensing in response to diamide and 2-methylhydroquinone (MHQ) in vivo. DNA-binding experiments indicate that YodB is regulated by a reversible thiol-modification in response to diamide and MHQ in vitro. Redox-regulation of YodB involves Cys6-Cys101' intermolecular disulfide formation by diamide and quinones in vitro. Diagonal Western blot analyses confirm the formation of intersubunit disulfides in YodB in vivo that require the conserved Cys6 and either of the C-terminal Cys101' or Cys108' residues. This study reveals a thiol-disulfide switch model of redox-regulation for the YodB repressor to sense electrophilic compounds in vivo.

References

May 1, 1992·Journal of Bacteriology·M WetzsteinW Schumann
Jan 1, 1992·Toxicology and Applied Pharmacology·T J MonksD G Graham
Jan 1, 1991·Chemico-biological Interactions·P J O'Brien
Dec 29, 1995·Gene·A M Guérout-FleuryP Stragier
Apr 16, 2002·Chemical Research in Toxicology·Yoshito KumagaiNobuhiro Shimojo
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mayuree Fuangthong, John D Helmann
May 8, 2002·Molecular Microbiology·Skorn MongkolsukSupa Utamapongchai
Jul 9, 2002·Molecular Microbiology·Skorn Mongkolsuk, John D Helmann
Dec 14, 2002·Biochemical and Biophysical Research Communications·Niroshini M GilesClaus Jacob
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shunji NakanoPeter Zuber
Nov 18, 2003·Annual Review of Genetics·Mark S B Paget, Mark J Buttner
Dec 8, 2004·Current Opinion in Structural Biology·David Barford
Oct 1, 2005·Molecular Microbiology·Falko HochgräfeMichael Hecker
Dec 2, 2006·Trends in Neurosciences·Takumi Satoh, Stuart A Lipton
Dec 13, 2006·Journal of Bacteriology·Montira Leelakriangsak, Peter Zuber
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin-Won LeeJohn D Helmann
Jul 6, 2007·The Journal of Biological Chemistry·Falko HochgräfeMichael Hecker
Dec 14, 2007·Environmental Science & Technology·Nopawan Ratasuk, Mark A Nanny
Feb 20, 2008·Expert Review of Proteomics·Haike AntelmannPeter Zuber
Apr 3, 2008·Environmental Science and Pollution Research International·Christian E W SteinbergRalph Menzel
Jul 1, 2008·Journal of Bacteriology·Sumarin SoonsangaJohn D Helmann
Aug 5, 2008·Molecular Microbiology·Manuel LiebekeHaike Antelmann
Jul 7, 2009·Nature Chemical Biology·Gerald L NewtonRobert C Fahey
Oct 20, 2009·Journal of Bacteriology·Dierk-Christoph PötherMichael Hecker
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ahmed GaballaJohn D Helmann

❮ Previous
Next ❯

Citations

Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Quanjiang JiChuan He
Jul 26, 2012·The Journal of Biological Chemistry·Bruno P LimaAlan J Wolfe
Jul 16, 2010·Antioxidants & Redox Signaling·Haike Antelmann, John D Helmann
Jan 13, 2012·Nucleic Acids Research·Gottfried J PalmHaike Antelmann
Apr 16, 2013·Journal of Bacteriology·Alexandra MüllerLars I Leichert
May 2, 2015·International Journal of Biological Macromolecules·Na CaoCheng-Ming Zeng
Jan 8, 2011·Archives of Biochemistry and Biophysics·Rong ChenCheng-Ming Zeng
Feb 27, 2015·Biological Chemistry·Melanie Hillion, Haike Antelmann
Aug 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sang Jae LeeBong-Jin Lee
Jan 15, 2017·Journal of the American Chemical Society·Guijie LiuCai-Guang Yang
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Jul 17, 2019·Antioxidants & Redox Signaling·Verena Nadin FritschHaike Antelmann
Mar 20, 2013·Molecular Microbiology·Changhan LeeChankyu Park
Feb 6, 2021·Biological Chemistry·Nico LinznerHaike Antelmann
Feb 20, 2021·Frontiers in Microbiology·Holly Anne MartinEduardo A Robleto
Apr 4, 2021·Antioxidants·In-Gyun Lee, Bong-Jin Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.