PMID: 4291787May 1, 1967Paper

The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver

The Biochemical Journal
D H WilliamsonH A Krebs

Abstract

1. The concentrations of the oxidized and reduced substrates of the lactate-, beta-hydroxybutyrate- and glutamate-dehydrogenase systems were measured in rat livers freeze-clamped as soon as possible after death. The substrates of these dehydrogenases are likely to be in equilibrium with free NAD(+) and NADH, and the ratio of the free dinucleotides can be calculated from the measured concentrations of the substrates and the equilibrium constants (Holzer, Schultz & Lynen, 1956; Bücher & Klingenberg, 1958). The lactate-dehydrogenase system reflects the [NAD(+)]/[NADH] ratio in the cytoplasm, the beta-hydroxybutyrate dehydrogenase that in the mitochondrial cristae and the glutamate dehydrogenase that in the mitochondrial matrix. 2. The equilibrium constants of lactate dehydrogenase (EC 1.1.1.27), beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were redetermined for near-physiological conditions (38 degrees ; I0.25). 3. The mean [NAD(+)]/[NADH] ratio of rat-liver cytoplasm was calculated as 725 (pH7.0) in well-fed rats, 528 in starved rats and 208 in alloxan-diabetic rats. 4. The [NAD(+)]/[NADH] ratio for the mitochondrial matrix and cristae gave virtually identical values in the same metaboli...Continue Reading

Citations

Jan 1, 1996·Critical Reviews in Clinical Laboratory Sciences·P W BakerR Bais
Oct 1, 1985·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·C Streffer
Nov 6, 2008·Expert Opinion on Drug Safety·Kalyanasundaram SubramanianSrivatsan Raghunathan
Oct 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation·V MacMillan, B K Siesjö
Nov 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation·B Hindfelt
Nov 1, 1993·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·C PoizatP Cuchet
Dec 1, 1979·Archives internationales de physiologie et de biochimie·M Feraudi
Jan 16, 2007·Molecular and Cellular Endocrinology·Daniel P SherbetRichard J Auchus
Jun 4, 2015·Ukrainian biochemical journal·Yu E KolupaevV A Khripach
Mar 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Hong ZhuWei Chen
Jul 24, 2012·Trends in Endocrinology and Metabolism : TEM·Liana Roberts Stein, Shin-ichiro Imai
Sep 1, 1992·Kidney International·A GougouxP Vinay
Nov 1, 1988·Kidney International·M Rengel-ArandaJ M Lopez-Novoa
Jun 1, 1995·Kidney International·N CanoX M Leverve
Oct 4, 2011·Progress in Neurobiology·Thomas Seifert, Niels H Secher
Jan 6, 2016·Aging Cell·Robert J MishurShane L Rea
Mar 23, 2011·Neurochemistry International·Paul C Engel
Jan 25, 2011·Chemico-biological Interactions·Claudia A StaabEdmund Maser
Nov 27, 2010·Neurochemistry International·Arthur J L Cooper
Jan 1, 2016·Cellular and Molecular Life Sciences : CMLS·Inês MesquitaRicardo Silvestre
Mar 24, 2010·Mechanisms of Ageing and Development·Ping Xu, Anthony A Sauve
Mar 2, 2010·Journal of Pharmacological and Toxicological Methods·Csaba CsonkaPéter Ferdinandy
Mar 30, 2010·Nuclear Medicine and Biology·Jason L J Dearling, Alan B Packard
Oct 2, 2014·Current Opinion in Biotechnology·Yuzheng Zhao, Yi Yang
Dec 18, 2014·Biophysical Journal·Michael A MoxleyJason N Bazil
Dec 17, 2008·Biochemical and Biophysical Research Communications·Gabriela SuchankovaNeil B Ruderman
Oct 13, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Silvia MangiaKâmil Uğurbil
Sep 11, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gerald A Dienel
Nov 29, 2008·Chemico-biological Interactions·Claudia A StaabJan-Olov Höög
Oct 28, 2015·Nature Reviews. Endocrinology·Keir J MenziesJohan Auwerx
Oct 1, 2008·Molecular and Cellular Endocrinology·Dario Mizrachi, Richard J Auchus
Sep 2, 2008·Molecular and Cellular Endocrinology·Andrew S MidzakBarry R Zirkin
Dec 28, 2007·Journal of Theoretical Biology·Enrique Meléndez-HeviaFrancisco Montero
Mar 10, 2016·Nature Reviews. Molecular Cell Biology·Evandro Fei FangVilhelm A Bohr
Nov 17, 2015·Biochimica Et Biophysica Acta·Andrei D Vinogradov, Vera G Grivennikova
Sep 24, 2015·Experimental Gerontology·Neil CopesPatrick C Bradshaw
Aug 12, 2005·Annals of the New York Academy of Sciences·Marco E CabreraGerald M Saidel

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