The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.

The Journal of Biological Chemistry
D A ArnoldS C Kowalczykowski

Abstract

Homologous recombination in Escherichia coli is initiated by the RecBCD enzyme and is stimulated by an 8-nucleotide element known as Chi (chi). We present a detailed biochemical characterization of a mutant RecBCD enzyme, designated RecBC1004D, that displays a reduced level of chi site recognition. Initially characterized genetically as unable to respond to the chi sequence, we provide evidence to indicate that the ability of this mutant enzyme to respond to chi is reduced rather than lost; the mutant displays about 20-fold lower chi recognition than wild-type RecBCD enzyme. Although this enzyme exhibits wild-type levels of double-stranded DNA exonuclease, helicase, and ATPase activity, its ability to degrade single-stranded DNA is enhanced 2-3-fold. The data presented here suggest that the reduced recombination proficiency of the recBC1004D strain observed in vivo results from a basal level of modification of the RecBC1004D enzyme at both chi-specific, as well as nonspecific, DNA sequences.

References

Aug 1, 1977·Genetics·F W Stahl, M M Stahl
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jul 26, 1991·Cell·D A Dixon, S C Kowalczykowski
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L J RomanS C Kowalczykowski
Sep 1, 1990·Genetics·S K AmundsenG R Smith
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J H LeBowitzR McMacken
Apr 20, 1987·Journal of Molecular Biology·K C Cheng, G R Smith
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S K AmundsenG R Smith
May 1, 1985·Cell·A S PonticelliG R Smith
Jul 1, 1966·The Journal of General Physiology·J Vinograd, J Lebowitz
Apr 1, 1971·Journal of Bacteriology·F Capaldo-Kimball, S D Barbour
Dec 5, 1984·Journal of Molecular Biology·K C Cheng, G R Smith
Jan 1, 1983·Methods in Enzymology·J Messing
Aug 1, 1983·Journal of Bacteriology·D W SchultzG R Smith
Nov 1, 1984·Journal of Bacteriology·A M Chaudhury, G R Smith
May 1, 1981·Cell·G R SmithK L Triman
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·A KöppenW Wackernagel
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·R S MyersF W Stahl
Jul 7, 1995·The Journal of Biological Chemistry·D A Dixon, S C Kowalczykowski
Sep 1, 1994·Microbiological Reviews·S C KowalczykowskiW M Rehrauer
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·D A DixonS C Kowalczykowski
Aug 1, 1997·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N HandaI Kobayashi
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·A J CLARK, A D MARGULIES

❮ Previous
Next ❯

Citations

Nov 13, 2004·Nature·Martin R SingletonDale B Wigley
Jul 25, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M El KarouiA Gruss
May 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Naofumi HandaStephen C Kowalczykowski
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Mark S Dillingham, Stephen C Kowalczykowski
May 29, 2014·PloS One·Ileabett M Echevarría-VargasPablo E Vivas-Mejía
Dec 9, 2014·Cell Cycle·Martin Wilkinson, Dale B Wigley
Dec 5, 2006·Journal of Molecular Biology·Naofumi Handa, Stephen C Kowalczykowski
Apr 4, 2017·Nucleic Acids Research·Luisa LauretiVincent Pagès
Sep 15, 1998·Journal of Molecular Biology·D G Anderson, S C Kowalczykowski
Aug 5, 2006·Journal of Molecular Biology·Barbara DziegielewskaPiero R Bianco
Jun 20, 2003·Nature·Mark S DillinghamStephen C Kowalczykowski
Apr 15, 2000·The Journal of Biological Chemistry·D A Arnold, S C Kowalczykowski
Jul 26, 2005·The Journal of Biological Chemistry·Maria SpiesStephen C Kowalczykowski
Sep 1, 2010·EcoSal Plus·Piero R Bianco

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.