The Reducing Capacity of Thioredoxin on Oxidized Thiols in Boiled Wort

Journal of Agricultural and Food Chemistry
Anne N MurmannMarianne N Lund

Abstract

Free thiol-containing proteins are suggested to work as antioxidants in beer, but the majority of thiols in wort are present in their oxidized form as disulfides and are therefore not active as antioxidants. Thioredoxin, a disulfide-reducing protein, is released into the wort from some yeast strains during fermentation. The capacity of the thioredoxin enzyme system (thioredoxin, thioredoxin reductase, NADPH) to reduce oxidized thiols in boiled wort under fermentation-like conditions was studied. Free thiols were quantitated in boiled wort samples by derivatization with ThioGlo1 and fluorescence detection of thiol-derivatives. When boiled wort was incubated with all components of the thioredoxin system at pH 7.0 and 25 °C for 60 min under anaerobic conditions, the free thiol concentration increased from 25 to 224 μM. At pH values similar to wort (pH 5.7) and beer (pH 4.5), the thioredoxin system was also capable of increasing the free thiol concentration, although with lower efficiency to 187 and 170 μM, respectively. The presence of sulfite, an important antioxidant in beer secreted by the yeast during fermentation, was found to inactivate thioredoxin by sulfitolysis. Reduction of oxidized thiols by the thioredoxin system was t...Continue Reading

References

Jul 30, 1990·FEBS Letters·M WürfelH Follmann
Oct 1, 1985·Journal of Biochemical and Biophysical Methods·N KumarJ E Kinsella
Feb 1, 1993·European Journal of Biochemistry·M WürfelH Follmann
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·S N Gorlatov, T C Stadtman
Feb 10, 2011·Journal of Agricultural and Food Chemistry·Daniel IntelmannThomas Hofmann
Oct 22, 2011·International Journal of Molecular Sciences·Ming J WuVincent J Higgins
Dec 20, 2011·Letters in Applied Microbiology·T S Berner, N Arneborg
May 1, 2012·Journal of Agricultural and Food Chemistry·Signe HoffMogens L Andersen
Jun 26, 2012·FEBS Letters·Johanne Mørch JensenBirte Svensson
Oct 4, 2012·Journal of Agricultural and Food Chemistry·Natália E C de AlmeidaDaniel R Cardoso
Feb 2, 2013·Journal of Agricultural and Food Chemistry·Signe HoffMarianne N Lund
Sep 7, 2013·Journal of Agricultural and Food Chemistry·Natália E C de AlmeidaDaniel R Cardoso

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