The reduction of nitrate to ammonium by a Clostridium sp. isolated from soil

Journal of General Microbiology
W H Caskey, J M Tiedje

Abstract

Cultures of Clostridium KDHS2 reduced 15NO3- to 15NH4+ with a concurrent increase in molar growth yield of 15.7% compared with fermentatively grown bacteria. The bacteria exhibited a Ks (NO3-) of 0.5 mM and reduced NO3- maximally at a rate of 0.1 mumol h(-1) mg dry wt)-1. A partially purified nitrate reductase was obtained which had a Km (NO3-) of 0.15 mM. The reduction of 13NO3- to 13NH4+ by resting bacteria was not inhibited by NH4+, glutamate, glutamine, methionine sulphoximine or azaserine. Glutamine synthetase affected neither the synthesis nor the activity of the NO3(-)-reducing enzymes. The results are consistent with the hypothesis that NO3- reduction to NH4+ in this Clostridium sp. is dissimilative. SO32-, but not SO42-, inhibited the reaction, apparently at the level of NO2- reduction.

Citations

Jan 1, 1982·Antonie van Leeuwenhoek·J M TiedjeJ A Robinson
Mar 1, 1982·Microbiological Reviews·R Knowles
Jul 6, 2014·Journal of Bioscience and Bioengineering·Li XieQi Zhou
Sep 21, 2017·Frontiers in Microbiology·Eveline M van den BergMark C M van Loosdrecht

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