PMID: 8939809Dec 1, 1996Paper

The red/white colony color assay in the yeast Saccharomyces cerevisiae: epistatic growth advantage of white ade8-18, ade2 cells over red ade2 cells

Current Genetics
S Ugolini, C V Bruschi

Abstract

In the yeast Saccharomyces cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment, while epistatic mutations in the same pathway, i.e. ade8, preclude this phenomenon, resulting in normal white colonies. The shift in color from red to white (or vice versa) with a combination of appropriate wild-type and mutant alleles of the adenine-pathway genes has been widely utilized as a non-selective phenotype to visualise and quantify the occurrence of various genetic events such as recombination, conversion and aneuploidy. It has provided an invaluable tool for the study of gene dosage and plasmid stability. In competition experiments between disrupted ade2, ade8-18 transformants carrying either a functional or non-functional episomal ADE8 gene, we verified that white ade8 ade2 cells show a remarkable selective advantage over red ade2 cells, with important implications on the use of this assay for the monitoring of genetic events. The accumulation of the red pigment in ade2 cells is likely to be the cause for impaired growth in these cells.

Citations

Sep 3, 2008·Molecular Genetics and Genomics : MGG·Katarzyna Tomala, Ryszard Korona
Aug 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Petr ObrdlikWolf B Frommer
Feb 11, 2016·Journal of Biotechnology·Jean Paul OuedraogoArthur F J Ram
Sep 11, 2013·Annual Review of Genetics·Stephanie KuengSusan M Gasser
May 8, 2018·Nature Biotechnology·Kevin R RoyLars M Steinmetz
Aug 26, 2017·Biotechnology Progress·Tatsuro Shibui, Hiroyoshi Hara
Feb 13, 2018·Applied Microbiology and Biotechnology·Luiza Cesca PivaFernando Araripe Gonçalves Torres
Jan 20, 2018·G3 : Genes - Genomes - Genetics·Emily RoggenkampGregory C Finnigan
May 28, 2019·Molecular Microbiology·Lakshmeesha Kempaiah NagappaHemalatha Balaram
Apr 23, 2008·Current Protocols in Neuroscience·Christopher GrefenPetr Obrdlik
Jun 7, 2012·G3 : Genes - Genomes - Genetics·Mark K Chee, Steven B Haase
Feb 23, 2007·Yeast·Ivan SadowskiJennifer Parent
May 31, 2017·Microbial Biotechnology·Irina S Druzhinina, Christian P Kubicek

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