The refined crystal structure of Bacillus cereus oligo-1,6-glucosidase at 2.0 A resolution: structural characterization of proline-substitution sites for protein thermostabilization

Journal of Molecular Biology
K WatanabeY Suzuki

Abstract

The crystal structure of oligo-1,6-glucosidase (dextrin 6-alpha-glucanohydrolase, EC 3.2.1.10) from Bacillus cereus ATCC7064 has been refined to 2.0 A resolution with an R-factor of 19.6% for 43,328 reflections. The final model contains 4646 protein atoms and 221 ordered water molecules with respective root-mean-square deviations of 0.015 A for bond lengths and of 3.166 degrees for bond angles from the ideal values. The structure consists of three domains: the N-terminal domain (residues 1 to 104 and 175 to 480), the subdomain (residues 105 to 174) and the C-terminal domain (residues 481 to 558). The N-terminal domain takes a (beta/alpha)8-barrel structure with additions of an alpha-helix (N alpha6') between the sixth strand Nbeta6 and the sixth helix N alpha6, an alpha-helix (N alpha7') between the seventh strand Nbeta7 and the seventh helix N alpha7 and three alpha-helices (N alpha8', N alpha8" and N alpha8'" between the eighth strand Nbeta8 and the eighth helix N alpha8. The subdomain is composed of an alpha-helix, a three-stranded antiparallel beta-sheet, and long intervening loops. The C-terminal domain has a beta-barrel structure of eight antiparallel beta-strands folded in double Greek key motifs, which is distorted in t...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Jun 1, 1992·European Journal of Biochemistry·S KimuraS Kanaya
Nov 15, 1991·The Biochemical Journal·H M JespersenB Svensson
Feb 20, 1991·Journal of Molecular Biology·C Klein, G E Schulz
Oct 1, 1991·Journal of Bacteriology·T KurikiT Imanaka
Mar 20, 1991·Journal of Molecular Biology·M W MacArthur, J M Thornton
Jun 1, 1989·Journal of General Microbiology·T Kuriki, T Imanaka
Jun 17, 1988·Science·J S Richardson, D C Richardson
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B W MatthewsW J Becktel
May 28, 1968·Journal of Molecular Biology·P R Schimmel, P J Flory
Mar 1, 1984·Journal of Biochemistry·Y MatsuuraM Kakudo
Jan 1, 1981·Advances in Protein Chemistry·J S Richardson
Apr 1, 1982·International Journal of Peptide and Protein Research·P Argos, J Palau
Feb 4, 1994·Journal of Molecular Biology·S B LarsonA McPherson
May 27, 1994·Journal of Molecular Biology·A KadziolaR Haser
Feb 1, 1993·Protein Engineering·T UedaT Imoto
Jan 23, 1987·Science·A T BrüngerM Karplus

❮ Previous
Next ❯

Citations

May 7, 2009·Applied Biochemistry and Biotechnology·Xue-Qiang WuFei Zou
Apr 12, 2012·The Journal of Biological Chemistry·Mònica Rius, Josep Chillarón
Nov 4, 2008·Journal of Biochemistry·Kazufumi TakanoShigenori Kanaya
Dec 17, 2008·Journal of Biochemistry·Jóhanna ArnórsdóttirMagnús M Kristjánsson
Sep 5, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Wataru SaburiAtsuo Kimura
Nov 11, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Keizo YamamotoShigeyoshi Osaki
Mar 6, 2012·Applied and Environmental Microbiology·Michael AnbarEdward A Bayer
Apr 14, 2007·BMC Bioinformatics·K MizuguchiM V Cubellis
May 28, 2010·BMC Structural Biology·Todd J Taylor, Iosif I Vaisman
Dec 8, 2006·Bioscience, Biotechnology, and Biochemistry·Jintanart WongchawalitAtsuo Kimura
Oct 26, 2001·Bioscience, Biotechnology, and Biochemistry·K WatanabeY Suzuki
Aug 9, 2011·Bioscience, Biotechnology, and Biochemistry·Momoko KobayashiAtsuo Kimura
Feb 20, 2013·BMC Biotechnology·Jirattikarn KaewmuangmoonChanpen Chanchao
Nov 13, 1998·Journal of Molecular Biology·T H TahirovI Kato
Jan 4, 2017·Biochemical and Biophysical Research Communications·Jong-Hyun JungCheon-Seok Park
Feb 12, 2017·International Journal of Food Microbiology·Susana DelgadoBaltasar Mayo
Apr 25, 2000·Protein Engineering·S KumarR Nussinov
Jul 29, 2017·The Journal of Organic Chemistry·Anais Vieira Da CruzSandrine Py
Oct 25, 2017·Interdisciplinary Sciences, Computational Life Sciences·Rajashekar Varma Kadumuri, Ramakrishna Vadrevu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.