PMID: 8947547Dec 1, 1996Paper

The refolding activity of the yeast heat shock proteins Ssa1 and Ssa2 defines their role in protein translocation

The Journal of Cell Biology
G L Bush, D I Meyer

Abstract

Ssa1/2p, members of one of the yeast cytosolic hsp70 subfamilies, have been implicated in the translocation of secretory proteins into the lumen of the ER. The involvement of these hsp70s in translocation was tested directly by examining the effect of immunodepleting Ssa1/2p from yeast cytosol and subsequently testing the cytosol for its ability to support co- and post-translational translocation of prepro-alpha-factor. Depletion of Ssa1/2p had no effect on the efficiency of translocation in this in vitro assay. The system was used to examine the effect of the absence of Ssa1/2p on two other putative hsp70 functions: cotranslational folding of nascent luciferase and refolding of denatured luciferase. Depletion of Ssa1/2p had no effect on the ability of the yeast lysate to synthesize enzymatically active luciferase, but had a dramatic effect on the ability of the lysate to refold chemically denatured luciferase. These results demonstrate, for the first time, the refolding activity of Ssa1/2p in the context of the yeast cytosol, and define refolding activity as a chaperone function specific to Ssa1/2p, aprt from other cytosolic hsp70s. They also suggest that Ssa1/2p do not play a significant role in chaperoning the folding of nas...Continue Reading

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Citations

Mar 27, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Li-Qiong XuSarah Perrett
Sep 26, 2009·PLoS Computational Biology·Xueping YuJaques Reifman
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·M GautschiS Rospert
Mar 28, 2013·Critical Reviews in Biochemistry and Molecular Biology·Tslil Ast, Maya Schuldiner
Sep 29, 2005·Biochemical and Biophysical Research Communications·Viviana VoltaStefano Biffo
Jan 15, 2011·The Biochemical Journal·Jurre HagemanHarm H Kampinga
Nov 9, 2001·Annual Review of Genetics·S W FewellJ L Brodsky
Sep 20, 2018·The Journal of Biological Chemistry·Weibin GongSarah Perrett
Nov 24, 1999·The Journal of Biological Chemistry·J NanduriA M Tartakoff
Sep 21, 2018·Frontiers in Molecular Neuroscience·Sebastian HoferDidac Carmona-Gutierrez
Aug 31, 2019·International Journal of Molecular Sciences·Alijah A Griffith, William Holmes

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