The Reg1-interacting proteins, Bmh1, Bmh2, Ssb1, and Ssb2, have roles in maintaining glucose repression in Saccharomyces cerevisiae.

The Journal of Biological Chemistry
Kenneth M DombekElton T Young

Abstract

In Saccharomyces cerevisiae, a type 1 protein phosphatase complex composed of the Glc7 catalytic subunit and the Reg1 regulatory subunit represses expression of many glucose-regulated genes. Here we show that the Reg1-interacting proteins Bmh1, Bmh2, Ssb1, and Ssb2 have roles in glucose repression. Deleting both BMH genes causes partially constitutive ADH2 expression without significantly increasing the level of Adr1 protein, the major activator of ADH2 expression. Adr1 and Bcy1, the regulatory subunit of cAMP-dependent protein kinase, are both required for this effect indicating that constitutive expression in Deltabmh1Deltabmh2 cells uses the same activation pathway that operates in Deltareg1 cells. Deletion of both BMH genes and REG1 causes a synergistic relief from repression, suggesting that Bmh proteins also act independently of Reg1 during glucose repression. A two-hybrid interaction with the Bmh proteins was mapped to amino acids 187-232, a region of Reg1 that is conserved in different classes of fungi. Deleting this region partially releases SUC2 from glucose repression. This indicates a role for the Reg1-Bmh interaction in glucose repression and also suggests a broad role for Bmh proteins in this process. An in vivo R...Continue Reading

References

Dec 11, 1991·Nucleic Acids Research·S Henikoff, J G Henikoff
Sep 1, 1991·European Journal of Biochemistry·D Niederacher, K D Entian
Jan 1, 1991·Methods in Enzymology·F Sherman
Jan 1, 1991·Proteins·G D SchulerD J Lipman
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
May 1, 1986·Genetics·R K Mortimer, J R Johnston
Jan 1, 1984·Molecular and Cellular Biology·J L Celenza, M Carlson
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·D GelperinS Lemmon
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·K LudinM Carlson
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·J M Gancedo
May 14, 1999·Current Opinion in Microbiology·M Carlson
Dec 23, 1999·The Journal of Biological Chemistry·J S SloanE T Young
May 4, 2000·Molecular Biology of the Cell·J J BonnerK Lea
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Jul 12, 2001·Yeast·M J van HemertH Y Steensma

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Citations

Mar 5, 2013·Current Genetics·J Horák
Jan 25, 2013·Molecular Biology of the Cell·Quyen L AohMara C Duncan
Sep 3, 2009·Genes & Development·Ulrike von PlehweSabine Rospert
Dec 21, 2004·European Journal of Biochemistry·Karin ElbingLena Gustafsson
Dec 11, 2007·Applied and Environmental Microbiology·Oscar MaestreJuan C Mauricio
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Jacob VergheseKevin A Morano
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·George M Santangelo
Feb 29, 2008·Annual Review of Genetics·Shadia ZamanJames R Broach
Sep 12, 2012·Genetics·James R Broach
Jan 18, 2012·The Journal of Cell Biology·Michel BecuweSébastien Léon
Aug 1, 2006·Biotechnology and Bioengineering·Steen Lund WestergaardJens Nielsen
Jul 25, 2015·FEMS Yeast Research·Ömur Kayikci, Jens Nielsen
Jun 19, 2008·FEMS Microbiology Reviews·Juana M Gancedo
Mar 17, 2010·Biochimica Et Biophysica Acta·Kristin PeiskerSabine Rospert
Dec 17, 2015·Molecular and Cellular Biology·Katherine A BraunElton T Young
Mar 15, 2005·Yeast
Aug 5, 2017·Nature Structural & Molecular Biology·Ying ZhangSabine Rospert
Oct 31, 2014·The Journal of Biological Chemistry·Pabitra K ParuaElton T Young
Oct 6, 2005·The Biochemical Journal·Karin ElbingMartin C Schmidt
Jul 12, 2020·Applied Microbiology and Biotechnology·Özge Kalender, Pınar Çalık
Aug 15, 2020·Frontiers in Bioengineering and Biotechnology·Beatriz VallejoAgustín Aranda
Jul 23, 2020·Bioscience, Biotechnology, and Biochemistry·Shinobu TakagiShinichi Ohashi

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