PMID: 16508210Mar 2, 2006Paper

The region-specific functions of two ubiquitin C-terminal hydrolase isozymes along the epididymis

Experimental Animals
Jungkee KwonK Wada

Abstract

We previously showed that gad mice, which are deficient for ubiquitin C-terminal hydrolase L1 (UCH-L1), have a significantly increased number of defective spermatozoa, suggesting that UCH-L1 functions in sperm quality control during epididymal maturation. The epididymis is the site of spermatozoa maturation, transport and storage. Region-specific functions along the epididymis are essential for establishing the environment required for sperm maturation. We analyzed the region-specific expression of UCH-L1 and UCH-L3 along the epididymis, and also assessed the levels of ubiquitin, which has specificity for UCH-L1. In wild-type mice, western blot analysis demonstrated a high level of UCH-L1 expression in the caput epididymis, consistent with ubiquitin expression, whereas UCH-L3 expression was high in the cauda epididymis. We also investigated the function of UCH-L1 and UCH-L3 in epididymal apoptosis induced by efferent duct ligation. The caput epididymides of gad mice were resistant to apoptotic stress induced by efferent duct ligation, whereas Uchl3 knockout mice showed a marked increase in apoptotic cells following ligation. In conclusion, the response of gad and Uchl3 knockout mice to androgen withdrawal suggests a reciprocal ...Continue Reading

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Citations

Mar 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Zhihua LiuPeter T Lansbury
May 25, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Peter Sutovsky
Nov 3, 2009·Progress in Neurobiology·Ian N M Day, Rod J Thompson
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Dec 27, 2012·Molecular Biology Reports·Cong-Cong Hou, Wan-Xi Yang
Aug 1, 2013·Physiological Reviews·Michael J ClagueSylvie Urbé

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