The regulated exocytosis of enlargeosomes is mediated by a SNARE machinery that includes VAMP4.

Journal of Cell Science
Emanuele CocucciJacopo Meldolesi

Abstract

The mechanisms governing the fast, regulated exocytosis of enlargeosomes have been unknown, except for the participation of annexin-2 in a pre-fusion step. We investigated whether any SNAREs are involved. In PC12-27 cells, which are enlargeosome-rich, the expressed SNAREs exhibited various distributions (trans-Golgi network, scattered puncta, plasma membrane); however, only VAMP4 was colocalized in discrete puncta with the enlargeosome marker desmoyokin. The exocytosis of the organelle, revealed by capacitance increases and by surface appearance of desmoyokin, was largely inhibited by microinjection of anti-VAMP4, anti-syntaxin-6 and anti-SNAP23 antibodies, by incubation with botulinum toxin E, and by transfection of VAMP4 and syntaxin-6 siRNAs. Microinjection of the antibodies anti-VAMP7, anti-VAMP8 and anti-syntaxin-4, and transfection with the VAMP8 siRNA were ineffective. Inhibition of enlargeosome exocytosis by VAMP4 siRNA also occurred in a cell type that was competent for neurosecretion, SH-SY5Y. Moreover, in cells expressing a VAMP4-GFP construct, enlargeosome exocytosis and surface appearance of fluorescence occurred concomitantly, and many ensuing surface patches were co-labelled by GFP and desmoyokin. VAMP4, an R-SNA...Continue Reading

References

Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·H KasaiJ Meldolesi
Jun 8, 1999·Molecular Biology of the Cell·M SteegmaierR H Scheller
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·M RupnikR Zorec
Mar 10, 2001·Nature·J B BockR H Scheller
Mar 14, 2001·American Journal of Physiology. Cell Physiology·A BanerjeeJ H Schwartz
Jun 16, 2001·Molecular Biology of the Cell·F WendlerS A Tooze
Sep 14, 2001·Traffic·F Wendler, S Tooze
Apr 6, 2002·Archives of Physiology and Biochemistry·H-P H MooreT E Machen
Jun 19, 2002·The Journal of Biological Chemistry·Christophe GrundschoberJacopo Meldolesi
Jun 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kira M S MisuraWilliam I Weis
Aug 29, 2002·Statistical Methods in Medical Research·Janet A ToozeRichard H Jones
Nov 26, 2002·Nature Cell Biology·Barbara BorgonovoJacopo Meldolesi
Jan 27, 2004·Molecular Biology of the Cell·Regina KuliawatPeter Arvan
Mar 3, 2004·The Journal of Biological Chemistry·Swathi K RaoNorma W Andrews
Oct 8, 2004·Molecular Biology of the Cell·Emanuele CocucciJacopo Meldolesi
Jan 7, 2005·Molecular Biology of the Cell·Sylvette Chasserot-GolazMarie-France Bader
Aug 25, 2005·The Journal of Biological Chemistry·Sanda A PredescuAsrar B Malik
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elena RealesAntonio Campos-Caro
Apr 19, 2006·The Journal of Cell Biology·Malika AhrasSharon A Tooze
Aug 17, 2006·Nature Reviews. Molecular Cell Biology·Reinhard Jahn, Richard H Scheller
Nov 2, 2006·Journal of Cell Science·Sestina FalconeJacopo Meldolesi
Nov 4, 2006·The EMBO Journal·Anna LorussoEvelina Chieregatti
Jan 12, 2007·Molecular Biology of the Cell·Cheng-Chun WangWanjin Hong
Mar 1, 2007·Journal of Cell Science·Ton Hoai Thi TranWanjin Hong
Apr 27, 2007·Biochemical and Biophysical Research Communications·Osamu KatsumataHiroshi Sugiya
Aug 31, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ignacio Delgado-MartínezJakob B Sørensen
Oct 20, 2007·The Journal of Biological Chemistry·Yiqun BaoWalter Hunziker
Jan 30, 2008·The Journal of Cell Biology·Dumaine Williams, Jeffrey E Pessin
Feb 27, 2008·Journal of Neurochemistry·Rosalba D'AlessandroJacopo Meldolesi

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Citations

Apr 25, 2012·Cell and Tissue Research·Kristian PrydzJaakko Saraste
May 28, 2013·Cancer Metastasis Reviews·Asfar S AzmiFazlul H Sarkar
Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Jun 27, 2013·Infection and Immunity·Emily J KabeisemanElizabeth R Moore
Sep 10, 2009·Cell Structure and Function·Miki OkayamaTaishin Takuma
Apr 2, 2013·Cellular and Molecular Life Sciences : CMLS·Glaucia N M HajjVilma R Martins
Aug 5, 2009·The Journal of Cell Biology·Zhuojin XuDzwokai Ma
Nov 24, 2012·Drug Delivery·Dimitry A ChistiakovAlexander N Orekhov
Aug 31, 2014·Cellular Signalling·T A DavisA-M Engelbrecht
May 22, 2009·Journal of Neurochemistry·Luisa StefanoJacopo Meldolesi
Aug 2, 2011·European Journal of Immunology·Konrad KrzewskiJack L Strominger
Oct 12, 2010·Mass Spectrometry Reviews·Matthias TrostPierre Thibault
May 11, 2011·The FEBS Journal·Kathleen Zylbersztejn, Thierry Galli
Jul 27, 2015·Biochimica Et Biophysica Acta·Eric Boucher, Craig A Mandato
May 31, 2012·Cellular Logistics·Denise M O Ramirez, Ege T Kavalali
Feb 19, 2011·Communicative & Integrative Biology·Rosalba D'AlessandroJacopo Meldolesi
Jul 7, 2010·Molecular Cell·Yujing ZhangChen-Yu Zhang
Nov 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Federico ColomboJacopo Meldolesi
Mar 8, 2016·Developmental Neurobiology·José Ignacio Wojnacki Fonseca, Thierry Galli
Jun 19, 2013·Nature Communications·Yang LiuZhen-Ge Luo
Aug 5, 2017·Nature Communications·Sicai ZhangPål Stenmark
Oct 18, 2012·Physiological Reviews·Haruo KasaiHiroshi Tokumaru
Dec 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yi PanKe Zen
Sep 23, 2020·Journal of Cellular Physiology·Bor L Tang
Feb 27, 2018·Journal of Neuromuscular Diseases·Florian BarthélémyMarc Bartoli
Nov 2, 2017·Developmental Neurobiology·Santiago QuirogaAlfredo Cáceres
Aug 12, 2014·Journal of Cell Science·Yang ZhouJunwei Yang
May 3, 2021·Progress in Neurobiology·Eric J R JansenGerard J M Martens
Aug 19, 2021·Nature Communications·Christine DeislMichael Fine

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