The regulation of alpha 5 beta 1 integrin expression in human muscle cells

Developmental Biology
K L Blaschuk, P C Holland

Abstract

The expression of alpha 5 beta 1 integrin was examined in either cloned or fluorescence-activated cell-sorted satellite cells derived from human biceps muscle. Removal of serum and factors required for muscle cell growth and proliferation both induced terminal differentiation and resulted in a coordinate downregulation of mRNA transcripts encoding alpha 5 and beta 1 integrin subunits. A corresponding downregulation of the alpha 5 subunit occurred at the protein level. Treatment of cultures with 5-bromo-2'-deoxyuridine (BUdR), a thymidine analog which inhibits muscle cell differentiation, resulted in increased expression of alpha 5 integrin subunit at both the mRNA and protein levels. However, levels of alpha 5 subunit message and protein were still markedly downregulated on removal of serum and growth factors from BUdR-treated cultures, indicating that downregulation of alpha 5 beta 1 integrin during myogenesis does not require and is not a consequence of muscle cell terminal differentiation. Downregulation of alpha 5 integrin subunit expression could be prevented by maintenance of cells in medium supplemented with serum and growth factors, although no single defined component of this medium could on its own prevent the downreg...Continue Reading

References

Sep 1, 1979·Experimental Cell Research·T R PodleskiK M Yamada
Oct 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C D MacCalmanO W Blaschuk
Oct 6, 1992·Biochimica Et Biophysica Acta·B SteffensenR J Klebe
Oct 1, 1990·Current Opinion in Cell Biology·K A Knudsen
Feb 1, 1990·Journal of Cellular Physiology·C Y Chung, M S Kang
Jul 1, 1989·The Journal of Cell Biology·R O HynesG H Yee
May 1, 1989·Differentiation; Research in Biological Diversity·K von der Mark, M Ocalan
Feb 1, 1989·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S ChampaneriaC Guérin
Sep 1, 1987·The Journal of Cell Biology·W S ArgravesE Ruoslahti
Aug 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R G HamH M Blau
Jan 1, 1988·Molecular Neurobiology·M D Schneider, E N Olson
Jan 1, 1988·Experimental Cell Research·C WebsterH M Blau
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H M Blau, C Webster
Jan 1, 1993·Developmental Biology·M I EnomotoA S Menko
Mar 1, 1993·Journal of Neuropathology and Experimental Neurology·G KarpatiP Holland

❮ Previous
Next ❯

Citations

Sep 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D GullbergT Sejersen
Sep 27, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M P WickJ C Sawdy
Sep 12, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Marni D BoppartStephen J Kaufman
Jul 21, 2007·American Journal of Respiratory Cell and Molecular Biology·Thai TranAndrew J Halayko
Sep 4, 2001·European Journal of Oral Sciences·M P LewisH L Tippett
Jul 23, 2014·Connective Tissue Research·Kelsey ThomasGretchen A Meyer
Mar 2, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ana Sofia CachaçoSólveig Thorsteinsdóttir
Sep 15, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Fernanda BajancaSólveig Thorsteinsdóttir
Jan 26, 2008·Trends in Molecular Medicine·Shihuan Kuang, Michael A Rudnicki
Jan 31, 2003·The Journal of Biological Chemistry·Ulrike Mayer
Mar 7, 1997·The Journal of Biological Chemistry·C PresottoG Salviati
Jul 18, 2019·American Journal of Physiology. Cell Physiology·Marni D Boppart, Ziad S Mahmassani
Dec 10, 1999·Experimental Cell Research·C HagiosR Chiquet-Ehrismann
May 18, 1999·Archives of Biochemistry and Biophysics·J E HanceM Michalak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.