The regulation of apoptosis by activin and transforming growth factor-beta in early neoplastic and tumorigenic ovarian surface epithelium

The Journal of Clinical Endocrinology and Metabolism
K C ChoiP C Leung

Abstract

Most ovarian neoplasms arise from the ovarian surface epithelium (OSE), and multiple growth factors have been implicated to influence the transformation from OSE. The present study was performed to investigate the role of activin and transforming growth factor-beta (TGFbeta) in normal and neoplastic OSE cells. An immortalized OSE cell line (IOSE-29) was generated from normal OSE by transfecting simian virus 40 large T antigen and was rendered tumorigenic after subsequent transfection with the E-cadherin gene (IOSE-29EC). The activin/inhibin subunits and activin receptors were expressed at both messenger ribonucleic acids and protein levels in these cells, suggesting that activin may have an autocrine role in neoplastic OSE cells. Treatments with activin (1-100 ng/mL) resulted in a significant decrease in cell proliferation in both IOSE-29 and IOSE-29EC cells, although we have shown that it stimulated the growth of ovarian cancer cells and had no effect on normal OSE. This inhibitory effect was attenuated with cotreatment with follistatin. Treatment with TGFbeta (0.1-10 ng/mL) also significantly decreased the proliferation of normal, IOSE-29, and IOSE-29EC cells in a dose-dependent manner. In addition, treatments with both activ...Continue Reading

Citations

Dec 3, 2011·Neural Plasticity·Jonas A Hosp, Andreas R Luft
Jun 17, 2015·Journal of Ovarian Research·Izabela WinklerJacek Tabarkiewicz
May 26, 2004·Differentiation; Research in Biological Diversity·Sarah L Maines-BandieraNelly Auersperg
Dec 16, 2004·International Review of Cytology·Hitoshi Okamura, Hidetaka Katabuchi
Jan 1, 2004·Molecular Endocrinology·Carolina J JorgezMartin M Matzuk
Dec 12, 2001·Endocrine Reviews·G P RisbridgerD M Robertson
Jun 12, 2004·Molecular Endocrinology·Dragana TomicJodi A Flaws
Oct 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Daniel C DanilaAnne Klibanski

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