The regulation of glucose transporter gene expression by cyclic adenosine monophosphate in NIH3T3 fibroblasts.

Molecular Endocrinology
Y HirakiM J Birnbaum

Abstract

The effect of cAMP on glucose transport was studied in fibroblastic cells. Incubation of confluent NIH3T3 cells for 6 h in the presence of cholera toxin (10 ng/ml) and 3-isobutyl-1-methylxanthine [(IBMX) 0.2 mM] or 8-bromo-cAMP (0.3 mM) and IBMX resulted in a 4-fold increase in the rate of deoxyglucose uptake; no change in hexose transport could be detected after treatment for 30 min. Either cholera toxin (0.3 ng/ml-30 ng/ml) or 8-bromo-cAMP (30 microM-3 mM) increased the expression of the mRNA encoding the glucose transporter (GT) protein, as determined by hybridization of size-fractionated total RNA to a rat brain GT cDNA. Activation of adenylate cyclase by forskolin also rapidly induced a 4- to 10-fold increase in GT mRNA. The rise in the level of GT mRNA was maximal 3-4 h after addition of the drug, and returned to basal values by 16 h. The stimulation was concentration dependent, with forskolin producing a maximal effect at 30 microM. The effect of a submaximal concentration (1 microM) of forskolin was greatly enhanced in the presence of IBMX (0.2 mM), which alone had little effect on GT mRNA levels. The forskolin-stimulated increase in GT mRNA was not blocked by inhibition of protein synthesis by cycloheximide (10 microgr...Continue Reading

Citations

Oct 12, 2000·Journal of Cellular Physiology·C J Clancey, J E Lever
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K H KaestnerM D Lane
Feb 1, 1994·European Journal of Biochemistry·M Mueckler
Dec 30, 2000·The Journal of Biological Chemistry·C ChenA Maity
Oct 1, 1992·Seminars in Nuclear Medicine·R A HawkinsL Seeger

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