The regulation of haemopoiesis in long-term bone marrow cultures: I. role of L-cell CSF

Journal of Cellular Physiology
T M Dexter, R K Shadduck

Abstract

The effects of L-cell conditioned medium which contains granulocyte/macrophage colony stimulating factor (CSF); of highly purified L-cell CSF; and the antiserum directed against L-cell CSF, have been investigated in long-term murine bone marrow cultures. Treatment of cultures with CSF containing conditioned medium led to a rapid decline in haemopoiesis. However, this inhibition of in vitro haemopoiesis is probably caused by materials other than CSF, since the addition of highly purified L-cell CSF had no appreciable effect upon long-term haemopoietic cell proliferation or differentiation. Furthermore, the inhibitory activity of L-cell conditioned medium was not abrogated following neutralization of the CSF activity by CSF antiserum. The direct addition of CSF antiserum did not inhibit granulocyte or macrophage formation. These results suggest that long-term cultures of murine marrow cells may show extensive interactions with stromal cells which are not influenced by exogenous stimulatory or inhibitory factors.

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Dec 1, 1977·Journal of Cellular Physiology·N WilliamsE M Rabellino
Sep 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·R K ShadduckE Gerard
Oct 7, 1976·Differentiation; Research in Biological Diversity·T D Allen, T M Dexter
Oct 1, 1975·Journal of Cellular Physiology·R K Shadduck, D Metcalf
Jun 1, 1966·The Australian Journal of Experimental Biology and Medical Science·T R Bradley, D Metcalf
Sep 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·R K ShadduckD R Boggs
Dec 1, 1971·Journal of Cellular Physiology·J W Sheridan, E R Stanley
Dec 1, 1971·The Journal of Clinical Endocrinology and Metabolism·J VaitukaitisG T Ross
Aug 1, 1970·Journal of Cellular Physiology·B L Pike, W A Robinson
Dec 1, 1965·Journal of Cellular Physiology·D H Pluznik, L Sachs

Citations

Apr 15, 1985·Klinische Wochenschrift·H G Mergenthaler
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K HarigayaR K Shadduck
Jun 3, 2010·Infection and Immunity·Eugenia Silva-Herzog, Corrella S Detweiler
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Songtao Shi, Stan Gronthos
Jan 1, 1989·Annals of the New York Academy of Sciences·P J QuesenberryD H Deacon
Jan 1, 1988·Critical Reviews in Oncology/hematology·H E Broxmeyer, D E Williams
Jan 1, 1981·Immunology Today·J W Schrader
Nov 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·O I Gan, N L Samoĭlina
Aug 15, 1987·Cancer·J L AscensaoM E Kaplan
Apr 1, 1992·Journal of Cellular Physiology·A UmezawaJ Hata
Jan 1, 1982·Journal of Cellular Physiology. Supplement·T M Dexter
May 1, 1984·Journal of Cellular Physiology·D Toksoz, G Brown
Jan 1, 1983·Journal of Cellular Physiology·R K ShadduckT M Dexter
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·D Zipori
Jan 1, 1980·Journal of Supramolecular Structure·R K ShadduckF Boegel

Related Concepts

Bone Marrow Cells
Protein Inducer MGI
Granulocyte
Hematopoiesis, Medullary
L Cells (Cell Line)

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