The regulation of membrane cofactor protein (CD46) expression by the 3' untranslated region in transgenic mice

Biochemical and Biophysical Research Communications
Shuji MiyagawaM Okabe

Abstract

Regulation of the membrane cofactor protein (MCP: CD46) was examined. While the expression of MCP in mice carrying MCP(BC2) cDNA with 125 bp of 3' untranslated region (3'UT) was minimal, that in mice carrying MCP cDNA without total 3' UT was evident in many organs. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis clearly showed the presence of mRNA even in transgenic mice with 3' UT, suggesting that the expression was regulated at the post-transcriptional stage. The in vitro expression data of MCP molecules on the stable Chinese hamster ovary (CHO) cell clone corresponded to that in transgenic mice. The first 125 bp downregulated the expression of MCP molecules in combination with not only beta-actin, but also SR alpha, promoter. Also, this region inhibited expression of decay accelerating factor (DAF: CD55) molecules when it was inserted into cDNA of DAF. Furthermore, the first 32 bp of the 3' UT revealed the same downregulation effect as 125 bp on MCP molecules. These findings indicated that the first 125 bp (and the first 32 bp in particular) of 3' UT regulate the expression of MCP molecules in transgenic mice.

References

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Citations

Jun 26, 2001·Clinical and Experimental Immunology·C KemperJ P Atkinson
Apr 2, 2002·Journal of Cardiac Surgery·M S SandrinI F McKenzie
Apr 2, 2002·Journal of Cardiac Surgery·S MiyagawaR Shirakura
Feb 13, 2010·Xenotransplantation·Shuji MiyagawaMasahiro Fukuzawa
Jun 14, 2003·Biochemical and Biophysical Research Communications·Daisuke FukutaRyota Shirakura
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masashi ShingaiTsukasa Seya
Nov 24, 1999·Biochemical and Biophysical Research Communications·M YoshitatsuR Shirakura

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