The regulation of mitogenesis and apoptosis in response to the persistent stimulation of alpha1-adrenoceptors: a possible role of 15-lipoxygenase

British Journal of Pharmacology
E Nishio, Y Watanabe

Abstract

1. Activation of alpha1-adrenoceptor stimulation regulates eicosanoid metabolism and growth in vascular smooth muscle cells (VSMCs). The purpose of this study was to investigate the functional implications of lipoxygenase pathway in alpha1-adrenoceptor-stimulated VSMCs growth through mutually exclusive biological functions, that is cell proliferation and cell death. 2. Phenylephrine (10 microM), a specific alpha1-adrenoceptor agonist, enhanced [3H]-thymidine incorporation by 300% above basal. Nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor, caused 36 and 50% decrease in phenylephrine (10 microM)-stimulated [3H]-thymidine incorporation at concentrations of 1 microM and 10 microM respectively. 3. Inversely, treatment of phenylephrine (10 microM)-stimulated VSMCs with NDGA induced DNA fragmentation in a dose-dependent fashion. The level of induction of DNA fragmentation by NDGA was 225, 319 and 406% above the phenylephrine (10 microM)-level at concentrations of 0.1 microM, 1 microM and 10 microM, respectively. This induction of DNA fragmentation was partially prevented by exogenous 15-hydroxyeicosatetraenoic acid (15-HETE). The inhibition of apoptosis was 53 and 63% at concentrations of 5 microM and 10 microM of 15HETE,...Continue Reading

References

Jan 1, 1992·Advances in Second Messenger and Phosphoprotein Research
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Ylä-HerttualaD Steinberg
Nov 1, 1990·Journal of Cellular Physiology·S HoshinaI B Weinstein
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Apr 27, 1995·Nature·M D Jacobson, M C Raff
May 1, 1995·The Journal of Clinical Investigation·M R BennettS M Schwartz
Jun 1, 1995·Circulation·J M IsnerJ Passeri
Aug 1, 1994·Atherosclerosis·M S Penn, G M Chisolm
Dec 11, 1994·Nucleic Acids Research·M HerrmannJ R Kalden
Nov 1, 1994·The Journal of Pharmacy and Pharmacology·M Qin, S Oie
Dec 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Holvoet, D Collen
Jan 1, 1994·Annual Review of Pharmacology and Toxicology·K P Minneman, T A Esbenshade
Jan 1, 1994·The Journal of Cell Biology·J C Reed
Jan 28, 1993·Nature·M J Berridge
Apr 5, 1996·Biochemical and Biophysical Research Communications·E NishioY Watanabe
Jun 14, 1996·Biochemical and Biophysical Research Communications·E NishioY Watanabe
Mar 1, 1997·The Journal of Clinical Investigation·H KühnC Gniwotta

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Citations

Aug 25, 2010·Infection and Immunity·Giuliana GiribaldiPaolo Arese
Jan 24, 2006·Infection and Immunity·Susanne Kirschnek, Erich Gulbins
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y CaoS M Prescott
Apr 13, 2013·Experimental Cell Research·G WeisingerN Stern
Aug 11, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Balaji MuralidharMark J Arends
Nov 13, 2007·Prostaglandins, Leukotrienes, and Essential Fatty Acids·I GátiN Dizdar

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