The regulation of Neuropilin 1 expression by miR-338-3p promotes non-small cell lung cancer via changes in EGFR signaling.

Molecular Carcinogenesis
Zongli DingJian-An Huang

Abstract

Neuropilin 1 (NRP1) is a transmembrane glycoprotein that acts as a co-receptor for multiple extracellular ligands and typically performs growth-promoting functions in cancer cells. Accumulating evidence indicates that NRP1 is upregulated, and may be an independent predictor of cancer relapse and poor survival, in many cancer types, including non-small cell lung cancer (NSCLC). Recent evidence suggests that NRP1 affects tumour cell viability via the epidermal growth factor receptor (EGFR) and Erb-B2 receptor tyrosine kinase 2 (ErbB2) signalling pathways in venous endothelial cells and in multiple cancer cells. In the present study, we aimed to evaluate the role of NRP1 in NSCLC tumourigenesis and to explore a new post-transcriptional mechanism of NRP1 regulation via a microRNA that mediates EGFR signalling regulation in lung carcinogenesis. The results showed that miR-338-3p is poorly expressed and NRP1 is overexpressed in NSCLC tissues relative to their levels in adjacent noncancerous tissues. Luciferase reporter assays, quantitative real-time reverse transcription PCR, and Western blot analyses showed that NRP1 is a direct target of miR-338-3p. Overexpression of miR-338-3p in NSCLC cell lines inhibited cell proliferation in vi...Continue Reading

References

Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Jun 10, 2005·Nature·Jun LuTodd R Golub
Jul 1, 2005·The New England Journal of Medicine·James L Mulshine, Daniel C Sullivan
Mar 15, 2006·Cancer Cell·Nozomu YanaiharaCurtis C Harris
Oct 10, 2006·Oncogene·M Jovanovic, M O Hengartner
Aug 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tse-Ming HongPan-Chyr Yang
Jul 16, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Chang-Qi ZhuUNKNOWN National Cancer Institute of Canada Clinical Trials Group Study BR.21
Jul 30, 2008·Cell·Joan Massagué
Oct 10, 2008·Cell Cycle·María José BuenoMarcos Malumbres
Mar 11, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Danit LebanonyMahesh Mansukhani
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Sep 6, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xiaogang TanJie He
Sep 19, 2012·Cancer Research·Sabrina RizzolioLuca Tamagnone
Oct 30, 2012·Nature Structural & Molecular Biology·Bert J C JanssenE Yvonne Jones
Jul 26, 2013·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Manish R PatelRobert A Kratzke
Jun 10, 2014·The Journal of Clinical Investigation·Chi-Hong ChaoChun-Ju Chang
Sep 11, 2014·Molecular and Cellular Biochemistry·Chunxi LiuWuwei Gu
Aug 8, 2015·International Journal of Oncology·Ying JinQingjie Ma
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Jul 1, 2016·Nature Communications·Haig AghajanianJonathan A Epstein
Mar 4, 2017·Journal of Cell Science·Lars MuhlUlf Eriksson
May 20, 2017·Molecular Cancer Therapeutics·Judith RaimbourgLisenn Lalier
Jul 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaoqiang ZhangYonggang Liang

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Citations

Oct 21, 2020·Journal of Cellular Physiology·Heng Wee TanAndy T Y Lau
Jan 10, 2021·Life Sciences·Sepideh MirzaeiMichael R Hamblin
Nov 13, 2020·Chinese Medical Journal·Shao-Dan LiuYong-Xiang Zhao
Apr 9, 2021·Cellular & Molecular Biology Letters·Meysam Moghbeli
Jul 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qian YiWeichao Sun

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Datasets Mentioned

BETA
GSE36681

Methods Mentioned

BETA
reverse
Assay
transfection
flow cytometry
xenograft

Software Mentioned

Image J
GEPIA
TargetScan
GraphPad Prism
Excel
SPSS
TargetScan Human

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