The regulation of primary mesenchyme cell migration in the sea urchin embryo: transplantations of cells and latex beads

Developmental Biology
C A Ettensohn, David R McClay

Abstract

After their ingression, the primary mesenchyme cells (PMCs) of the sea urchin embryo migrate within the blastocoel, where they eventually become arranged in a characteristic ring-like pattern. To gain information about how the movements of the PMCs are regulated, a microinjection procedure was developed and used to transplant PMCs to novel environments within embryos of different developmental stages. Donor PMCs were vitally stained with rhodamine isothiocyanate so their movements could be followed. When PMCs were transplanted into the blastocoel of embryos at the mesenchyme blastula stage, the donor cells moved to the vegetal region and joined with the host's PMCs in forming a normal ring pattern and skeleton. When PMCs were microinjected into the blastocoel of early (prehatching) blastulae, they moved toward and accumulated in the vegetal region of the host embryo within 3 hr. They did not, however, elaborate a complete ring pattern when they would have done so if left in situ. Instead, they participated in the formation of the ring pattern that was subsequently formed by the PMCs of the host embryo. These data indicate that information present in the host embryo and established well before the mesenchyme blastula stage guide...Continue Reading

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Citations

Aug 1, 1994·Developmental Biology·K M Malinda, C A Ettensohn
Feb 1, 1988·Developmental Biology·C A Ettensohn, D R McClay
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