PMID: 11918080Mar 29, 2002Paper

The regulation of prostate cancer cell adhesion to human bone marrow endothelial cell monolayers by androgen dihydrotestosterone and cytokines

Clinical & Experimental Metastasis
C R CooperKenneth J Pienta

Abstract

A previous study from our laboratory suggested that prostate cancer metastasis to bone may be mediated, in part, by preferential adhesion to human bone marrow endothelial (HBME) cells. Tumor cell adhesion to endothelial cells may be modulated by the effect of cytokines on cell adhesion molecules (CAMs). Tumor necrosis factor-alpha (TNF-alpha) regulates VCAM expression on the endothelium and this effect is enhanced by dihydrotestosterone (DHT). Transforming growth factor-beta (TGF-beta) stimulates the expression of alpha2beta1 integrin on PC-3 cells. The current study investigated the effects of the above cytokines and DHT (singularly and in various combinations) upon HBME and prostate cancer cell expression of VCAM, alpha2 integrin subunit, and beta1 integrin subunit by flow cytometry. We also monitored the effects of the above treatments on PC-3 cell adhesion to HBME monolayers. The data demonstrate that none of the treatments significantly altered the expression of selected CAMs on HBME cell and neoplastic prostate cell lines. The treatment of HBME monolayers with various combinations of cytokines and DHT prior to performing adhesion assays with PC-3 demonstrates that treatments containing TGF-beta reduced PC-3 cell adhesion ...Continue Reading

Citations

Dec 16, 2006·Cancer Metastasis Reviews·Vladislav V Glinsky
Nov 6, 2003·Clinical Orthopaedics and Related Research·Peter F M Choong
Dec 12, 2007·Cancer Metastasis Reviews·Karen M BussardAndrea M Mastro
Mar 21, 2009·In Vitro Cellular & Developmental Biology. Animal·Roman Paduch, Piotr Niedziela
Jan 28, 2003·Cancer·Carlton R CooperKenneth J Pienta
May 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shahrokh F ShariatKevin M Slawin
Oct 2, 2007·Clinical & Experimental Metastasis·Fayth L MilesCarlton R Cooper
Dec 14, 2006·Cancer Metastasis Reviews·Jianhua WangRussell S Taichman

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