The regulation of transcriptome responses in zebrafish embryo exposure to triadimefon

Environmental Toxicology
Li-Sung HsuSsu Ching Chen

Abstract

The residue of triadimefon (TDF) (a pesticide) has become the pollutant in water due to its intensive use in agriculture and medicine, and its stability in water leaching from soil and vegetation. In this study, RNA-seq, a high-throughput method was performed, to analyze the global expression of differential expressed genes (DEGs) in zebrafish embryos treated with TDF (10 μg/mL) from fertilization to 72 h post-fertilization (hpf) as compared with that in the control group (without TDF treatment). Two cDNA libraries were generated from treated and non-treated embryos, respectively. With the 79.4% and 78.8% of reads mapped to the reference, it was observed that many differential genes were expressed between the two libraries. The most 20 differentially expressed up-regulated or down-regulated genes were involving in the signaling transduction, the activation of many genes related to cytochrome P450 enzymes, and molecular metabolism. Validation of seven genes expression confirmed RNA-seq results. The transcriptome sequences were further subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and showed diverse biological functions and metabolic pathways. The data from this study contributed to a...Continue Reading

References

Jan 28, 2004·Annual Review of Pharmacology and Toxicology·James E Klaunig, Lisa M Kamendulis
Feb 3, 2005·Nature Reviews. Drug Discovery·Leonard I Zon, Randall T Peterson
Jun 15, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Richard C PefferIvan Rusyn
Jul 31, 2008·BMC Evolutionary Biology·Carlos E Alvarez
Apr 14, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stephen NesnowSusan D Hester
Jun 24, 2010·Toxicology Letters·Aihua GuXinru Wang
May 4, 2011·Environmental and Molecular Mutagenesis·Jong-C WuSsu Ching Chen
Oct 19, 2011·Environmental Toxicology and Pharmacology·Shaoying LiuGuonian Zhu
Jan 25, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Lindsey V T Garner, Richard T Di Giulio
Jun 6, 2012·Reproductive Toxicology·Sanne A B HermsenAldert H Piersma
Jun 6, 2012·Reproductive Toxicology·Joshua F RobinsonAldert H Piersma
Aug 28, 2012·Neuroscience Letters·Jyotshna KanungoSyed F Ali
Mar 19, 2013·Journal of Hazardous Materials·Zhao Sheng ZhouZhi Min Yang
Apr 27, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Eva OliveiraBenjamin Piña
Jul 20, 2014·Development·Hillary F McGrawAlex V Nechiporuk

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Citations

Oct 28, 2016·Environmental Toxicology·Tsung-Cheng ChuehSsu Ching Chen
Apr 5, 2017·International Journal of Molecular Sciences·Javiera F De la PazMiguel L Allende
Mar 18, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·You-Cheng HseuSsu Ching Chen
Feb 11, 2018·Environmental Toxicology·Heng-Dao LinSsu-Ching Chen
Sep 29, 2017·Environmental Science and Pollution Research International·Jinhua JiangLiezhong Chen
Nov 2, 2019·Environmental Toxicology·Christopher L SoudersChristopher J Martyniuk
Feb 20, 2020·Bulletin of Environmental Contamination and Toxicology·Yuqiong WuXianbiao Jiang

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