The regulator RamA influences cmytA transcription and cell morphology of Corynebacterium ammoniagenes.

Current Microbiology
Seok-Myung LeeHeung-Shick Lee

Abstract

RamA plays a regulatory role for acetate utilization and S-layer biosynthesis in Corynebacterium glutamicum. Looking for any additional role, the function of RamA was analyzed in Corynebacterium ammoniagenes, which is closely related to C. glutamicum. In this study, we showed that the DeltaramA mutant constructed by a markerless knockout strategy possessed increased cell surface hydrophobicity, leading to the formation of aggregated cell masses in liquid media. In addition, the mutant exhibited an elongated cell shape as observed by SEM, suggesting that cell wall-associated proteins might be influenced. Furthermore, cell surface proteome analysis revealed that the expression of cmytA gene encoding corynomycoloyl transferase required for cell wall biosynthesis was down-regulated in the mutant, supporting the regulatory role of RamA in cell wall assembly. These studies support a novel regulatory role of RamA in inducing the expression of proteins required for cell wall assembly.

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S G GrantD Hanahan
Jul 1, 1993·Journal of Bacteriology·M T FollettieA J Sinskey
Dec 26, 2001·Biochimica Et Biophysica Acta·Y UsudaT Utagawa
Apr 16, 2002·Molecular Microbiology·R Allyn ForsythJudith W Zyskind
Nov 5, 2005·Applied and Environmental Microbiology·Petra Peters-WendischLothar Eggeling
Jul 3, 2007·Metabolic Engineering·Soo-Dong ParkHeung-Shick Lee
Nov 4, 2008·Nucleic Acids Research·Ivica LetunicPeer Bork

❮ Previous
Next ❯

Citations

Jun 16, 2011·Proteomics·Ansgar PoetschAndreas Burkovski
May 29, 2018·Applied Microbiology and Biotechnology·Adnan ShahBernhard J Eikmanns

❮ Previous
Next ❯