PMID: 8606152Apr 1, 1996Paper

The regulatory characteristics of yeast fructose-1,6-bisphosphatase confer only a small selective advantage

Journal of Bacteriology
M A Navas, Juana M Gancedo

Abstract

The question of how the loss of regulatory mechanisms for a metabolic enzyme would affect the fitness of the corresponding organism has been addressed. For this, the fructose-1,6-bisphosphatase (FbPase) from Saccharomyces cerevisiae has been taken as a model. Yeast strains in which different controls on FbPase (catabolite repression and inactivation; inhibition by fructose-2,6-bisphosphate and AMP) have been removed have been constructed. These strains express during growth on glucose either the native yeast FbPase, the Escherichia coli FbPase which is insensitive to inhibition by fructose-2,6-bisphosphate, or a mutated E. coli FbPase with low sensitivity to AMP. Expression of the heterologous FbPases increases the fermentation rate of the yeast and its generation time, while it decreases its growth yield. In the strain containing high levels of an unregulated bacterial FbPase, cycling between fructose-6-phosphate and fructose-1,6-bisphosphate reaches 14%. It is shown that the regulatory mechanisms of FbPase provide a slight but definite competitive advantage during growth in mixed cultures.

References

Feb 24, 1989·Cell·B J Thomas, R Rothstein
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M SedivyD G Fraenkel
Apr 1, 1966·Archives of Biochemistry and Biophysics·D G FraenkelB L Horecker
Aug 1, 1980·European Journal of Biochemistry·S FunayamaC Gancedo
Aug 1, 1982·Molecular and Cellular Biology·M Bañuelos, D G Fraenkel
Jan 1, 1983·Methods in Enzymology·G Ammerer
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Sep 1, 1983·Archives of Biochemistry and Biophysics·J Babul, V Guixé
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M A NavasJ M Gancedo

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Citations

Oct 29, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Johan H van HeerdenBas Teusink
Oct 15, 2014·Biotechnology and Bioengineering·Thomas M Wasylenko, Gregory Stephanopoulos
Jun 14, 2002·The Journal of Biological Chemistry·Paula PortelaSilvia Rossi
Sep 14, 2013·Journal of Industrial Microbiology & Biotechnology·Jianzhong XuWeiguo Zhang
Sep 30, 2017·FEMS Yeast Research·Juana M Gancedo, Carlos Gancedo
Jul 14, 2009·Applied and Environmental Microbiology·Léonie G M BoenderJack T Pronk

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