Abstract
The epithelial-mesenchymal transition (EMT) plays a critical role in cancer progression, metastasis and drug resistance. The transcription factor(TF) and microRNA (miR) chimeric [SNAIL/miR-34]:[ZEB/miR-200] unit is the core regulatory system for the EMT process. Here, we proposed to assess the anti-EMT abilities and explore the inherent pharmacological mechanisms of the classic hypoglycaemic agent metformin for colorectal cancer(CRC). For the EMT model, the TGF-β-induced CRC cell lines SW480 and HCT116 were treated with metformin. The viability, migration and invasion abilities of the cells were evaluated with the Cell Counting Kit-8, wound-healing and trans-well assay. The alterations of the [SNAIL/miR-34]:[ZEB/miR-200] system and the EMT markers E-cadherin and vimentin were detected by western blot, qPCR and immunofluorescent staining. Metformin exhibited inhibitory effects on the proliferation, migration and invasion of the CRC SW480 cells. The up-regulation of E-cadherin and the down-regulation of vimentin for both SW480 and HCT116 cells revealed the anti-EMT abilities of metformin. For the [SNAIL/miR-34]:[ZEB/miR-200] system, metformin increased miR-200a, miR-200c and miR-429 levels and decreased miR-34a, SNAIL1 and ZEB1 l...Continue Reading
Citations
Feb 12, 2019·Oncogene·Guang-Xi ZhaoShe Chen
Mar 6, 2019·Molecular Biology Reports·Xiu-Lian Wang, Chao Huang
May 28, 2019·Medicinal Research Reviews·Ya-Long FengYing-Yong Zhao
Feb 6, 2020·Animals : an Open Access Journal From MDPI·Nakita CâmaraPedro Herráez
Apr 6, 2020·Journal of Cellular and Molecular Medicine·Fang ZhengSwei Sunny Hann
Feb 20, 2019·International Journal of Molecular Sciences·Dongsong NieJunjiang Fu
Dec 10, 2019·Cell Cycle·Yangmei ZhangKai Chen
Jul 20, 2019·Molecular Medicine Reports·Na LiCaixia Liu
Apr 19, 2020·Bioengineered·Hongwei WangRenyi Chen
Dec 12, 2018·Journal of Cellular Physiology·Chao HuangZuoliang Pang
Feb 14, 2021·Experimental Hematology & Oncology·Bing DongKongming Wu
Feb 12, 2021·Pharmaceuticals·Eunsun JungTae-Su Han
Jan 5, 2021·World Journal of Clinical Cases·Qi-You HuangQing-Ming Wu
Jan 17, 2021·Advances in Biological Regulation·Stephen L AbramsJames A McCubrey
Feb 23, 2021·European Journal of Pharmacology·Michelle Jaromy, Joshua D Miller
Sep 12, 2020·BMJ Open Respiratory Research·Zhenlin LiOnnik Agbulut
Apr 15, 2021·Cancer Cell International·Nahid AlimoradiReihaneh Fatehi
Apr 18, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mei-Tsz SuPao-Lin Kuo
May 9, 2021·Analytical Biochemistry·Bin WenKui Zhao
Jul 25, 2021·Cancers·Hee Eun KangTae Il Kim
Sep 9, 2021·Bioscience Reports·Jiahui WeiJiabao Zhang