Abstract
The small regulator SipA, interacts with the ATP-binding domain of non-bleaching sensor histidine kinase (NblS), the most conserved histidine kinase in cyanobacteria. NblS regulates photosynthesis and acclimation to a variety of environmental conditions. We show here that SipA is a highly stable protein in a wide pH range, with a thermal denaturation midpoint of 345 K. Circular dichroism and 1D 1H NMR spectroscopies, as well as modelling, suggest that SipA is a beta-II class protein, with short strands followed by turns and long random-coil polypeptide patches, matching the SH3 fold. The experimentally determined m-value and the heat capacity change upon thermal unfolding (DeltaCp) closely agreed with the corresponding theoretical values predicted from the structural model, further supporting its accuracy.
References
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Jan 1, 1986·Methods in Enzymology·C N Pace
Aug 1, 1995·Proteins·J GómezE Freire
Jan 1, 1995·Methods in Molecular Biology·C A Royer
Apr 27, 1993·Biochemistry·J Clarke, A R Fersht
Oct 1, 1995·Protein Science : a Publication of the Protein Society·J K MyersJ M Scholtz
Dec 22, 1999·Biochemistry·D K WilkinsL J Smith
Jan 23, 2003·Protein Science : a Publication of the Protein Society·Narasimha Sreerama, Robert W Woody
Jun 26, 2003·Nucleic Acids Research·Leigh WillardDavid S Wishart
Jun 26, 2003·Nucleic Acids Research·Narayanan EswarAndrej Sali
Jun 26, 2003·Protein Science : a Publication of the Protein Society·M Isabel Muro-PastorJosé L Neira
Jul 3, 2003·Journal of Bacteriology·J Alejandro D'Aquino, Dagmar Ringe
Jun 25, 2004·Nucleic Acids Research·Lee Whitmore, B A Wallace
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Feb 3, 2006·FEMS Microbiology Letters·Javier EspinosaAsunción Contreras
Apr 7, 2006·Protein Science : a Publication of the Protein Society·Richard L ThurlkillC Nick Pace
Sep 27, 2007·Biopolymers·Lee Whitmore, B A Wallace
Nov 17, 2007·Molecular Microbiology·Paloma SalinasAsunción Contreras
Oct 4, 2008·Genes & Development·Alexander Y Mitrophanov, Eduardo A Groisman
Dec 23, 2008·Journal of Molecular Biology·Matthew J BickSeth A Darst
Jan 28, 2009·Biochemistry·José L NeiraMiguel Vidal
Jun 2, 2009·Nucleic Acids Research·Kevin Karplus
Jun 23, 2009·Plant & Cell Physiology·Tasuku SakayoriIwane Suzuki
Oct 6, 2009·Cell·Patricia CasinoAlberto Marina
Citations
Mar 3, 2011·PloS One·David Aguado-LleraJosé L Neira
Jan 24, 2013·FEBS Letters·Félix Moronta-BarriosAsunción Contreras
Feb 7, 2012·Biochimica Et Biophysica Acta·Javier EspinosaAsunción Contreras
Oct 12, 2010·FEBS Letters·David Aguado-LleraJosé L Neira
Oct 28, 2010·Molecular Microbiology·Maria Luisa López-RedondoAsunción Contreras
Feb 11, 2012·Microbiology·Félix Moronta-BarriosAsunción Contreras
Apr 3, 2020·Biochimica Et Biophysica Acta. General Subjects·Clara Díaz-GarcíaJosé L Neira