The regulatory mechanism of Tremella mesenterica on steroidogenesis in MA-10 mouse Leydig tumor cells

Life Sciences
Yen-Wen ChenBu-Miin Huang

Abstract

Tremella mesenterica (TM), a yellow jelly mushroom, has been traditionally used as tonic food to improve body condition in Chinese society for a long time. We have previously demonstrated that TM reduced in vitro hCG-treated steroidogenesis in MA-10 mouse Leydig tumor cells without any toxicity effect. In the present study, the mechanism how TM suppressed hCG-treated steroidogenesis in MA-10 cells was investigated. MA-10 cells were treated with vehicle, human chorionic gonadotropin (hCG, 50 ng/ml), or different reagents with or without TM to clarify the effects. TM significantly suppressed progesterone production with the presences of forskolin (10 and 100 microM) or dbcAMP (0.5 and 1mM), respectively, in MA-10 cells (p<0.05), which indicated that TM suppressed steroidogenesis after PKA activation along the signal pathway. Beyond our expectation, TM induced the expression of steroidogenic acute regulatory (StAR) protein with or without hCG treatments. However, TM profoundly decreased P450 side chain cleavage (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) enzyme activities without any influences on the expression of both enzymes. These inhibitions on steroidogenic enzyme activities might counteract the stimulation ...Continue Reading

References

Mar 1, 1979·Molecular and Cellular Endocrinology·E R Simpson
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Sep 1, 1995·Biology of Reproduction·B M HuangR L Norman
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Feb 25, 2003·Life Sciences·Liang-Yu ChenBu-Miin Huang
Sep 24, 2004·The International Journal of Biochemistry & Cell Biology·Yung-Chia ChenBu-Miin Huang

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Citations

Dec 7, 2007·Toxicology Letters·Jian-Hua QuXin-Ru Wang

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