Abstract
A non-hydrolysable guanosine nucleotide analog, GTP[S] at 200 microM, stimulated amylase secretion which was inhibited by an anti-phospholipase A2 (PLA2) antibody in permeabilized pancreatic acini, indicating that the PLA2 pathway is linked to the GTP binding protein. A high affinity cholecystokinin (CCK) receptor agonist, CCK-OPE (10 microM), and a low affinity receptor agonist, CCK-8 (0.1 microM), both caused amylase secretion in permeabilized cells. The action of CCK-OPE was abolished by the G beta antibody but not by the G alpha-q,11 antibody, whereas the opposite was true of the CCK-8 response. Biscoclaurine alkaloid isotetrandrine (10 microM), a specific inhibitor of PLA2-coupled G proteins, abolished Ca2+ oscillations and amylase secretion induced by CCK-OPE (0.1-100 nM), but not by CCK-8 (10 pM) in intact acini. Gp antagonist-2A (10 microM), which inhibits the activation of Gq, also inhibited the actions of CCK-OPE (10 pM-1 microM) in intact acini. These observations indicate that the functional unit of the heterotrimeric G protein coupled to the high affinity CCK receptor appears to be different from that linked to the low affinity CCK receptor/Gq-alpha pathway. The regulatory site of this G protein coupled to the high...Continue Reading
Citations
Feb 10, 2004·Molecular Biology of the Cell·Diane ChanWilliam J Brown
May 27, 2011·Journal of Biochemistry·Jae Hyung BachDae Kyong Kim
Jul 15, 1996·The Journal of Clinical Investigation·R M KahnA D Fryer
Apr 5, 2003·Journal of Neurochemistry·Blandine PommierFlorence Noble
May 25, 2013·Biochimie·Maria Grazia SignorelloGiuliana Leoncini
Mar 17, 2009·Biochemical and Biophysical Research Communications·S Mahmoud A Najafi
Mar 14, 2003·FEBS Letters·Kwang Kook LeeMyoung Kyu Park
May 1, 1997·Endocrinology·Y YajimaS Kawashima
Jan 12, 2000·Pancreas·N MizunoT Hayakawa
Jun 20, 1997·The Journal of Biological Chemistry·H OhnishiJ A Williams
Feb 20, 2020·American Journal of Physiology. Cell Physiology·Cody W KowalskiJames H Peters
Apr 13, 1999·The American Journal of Physiology·F NozuC Owyang
Jun 5, 2002·World Journal of Gastroenterology : WJG·Yi YuZhi-Yong Dong
Jul 1, 1999·Journal of Neurochemistry·B PommierF Noble
Jan 31, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christelle GuibertJean-Pierre Savineau
Sep 6, 1996·The Journal of Biological Chemistry·T J Shuttleworth
May 23, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tim O LankischChung Owyang
Sep 1, 1997·Archives of Biochemistry and Biophysics·A CapdevilaC Wagner
May 18, 2001·General and Comparative Endocrinology·B B GuptaL Vollrath
Jul 4, 1998·European Journal of Pharmacology·F QuartulliB Pipy
Jul 29, 2000·Bone·S C RawlinsonL E Lanyon
Jan 17, 2009·The American Journal of Pathology·Ming Yan XuGisli Jenkins